Hohbadel D C, McNeely M D, Sunderman F W
Ann Clin Lab Sci. 1975 Jan-Feb;5(1):65-70.
An automated micromethod is described for measurement of serum ceruloplasmin by assay of its p-phenylenediamine oxidase activity using the Abbott bichoromatic analyzer. Ten mul of serum are mixed with 250 mul of p-phenylenediamine (PPD) substrate (9.2 mmole per liter) in acetate buffer (0.1 mole per liter, pH 5.45). Spectrophotometric measurements of the rate of formation of the purple oxidation porduct of PPD are performed after a 10 min delay for thermal equilibration at 37 degrees and for avoidance of the lag-phase of the enzymatic reaction. The coefficients of variation of replicate analyses of normal serum by this technique are 1.1 percent (within-the-run) and 3.3 percent (day-to-day). Measurements of ceruloplasmin concentrations in serums from 75 patients by this automated method provided close correlation with measurements by a manual reference procedure (correlation coefficient=0.973). The mean concentration of ceruloplasmin in serums from 64 healthy men was 29 mg per dl (central 95th percentile limits=22 to 40 mg per dl).
本文描述了一种自动化微量方法，用于通过使用雅培双波长分析仪检测对苯二胺氧化酶活性来测定血清铜蓝蛋白。将10微升血清与250微升对苯二胺（PPD）底物（9.2毫摩尔/升）在醋酸盐缓冲液（0.1摩尔/升，pH 5.45）中混合。在37℃下进行10分钟的热平衡并避免酶促反应的滞后阶段后，对PPD紫色氧化产物形成速率进行分光光度测量。用该技术对正常血清进行重复分析的变异系数为1.1%（批内）和3.3%（日间）。用这种自动化方法对75例患者血清中铜蓝蛋白浓度的测量结果与手动参考方法的测量结果密切相关（相关系数 = 0.973）。64名健康男性血清中铜蓝蛋白的平均浓度为29毫克/分升（第95百分位数范围 = 22至40毫克/分升）。
