Martínez-Subiela S, Tecles F, Ceron J J
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, Campus de Espinardo, 30100 Espinardo, Murcia, Spain.
Res Vet Sci. 2007 Aug;83(1):12-9. doi: 10.1016/j.rvsc.2006.10.011. Epub 2006 Dec 6.
Ceruloplasmin (Cp) determination could provide an objective measure of the health status of an animal and could be used as marker of animal health and welfare (Skinner, 2001) (Skinner, J. G., 2001. Special report. International standardization of acute phase proteins. Veterinary Clinical Pathology 30, 2-7.) but only manual methods have been reported to determine Cp concentrations in pigs (McCosker, 1961; Toussaint et al., 1995; Eckersall et al., 1996) (McCosker, P. J., 1961. Paraphenylenediamine oxidase activity and copper-levels in mammalian plasmas. Nature. 190, 887-889; Toussaint, M. J. M., Van Ederen, A. M., Gruys, E., 1995. Implication of clinical pathology in assessment of animal health and in animal production and meat inspection. Comparative Haematology International 5, 149-157; Eckersall, P. D., Saini, P. K., McComb, C., 1996. The acute phase response of acid soluble glycoprotein, alpha(1)-acid glycoprotein, ceruloplasmin, haptoglobin and C-reactive protein, in the pig. Veterinary Immunology and Immunopathology 51, 377-385). In the present study two automated methods based on the use of two different substrates for the determination of serum ceruloplasmin in pigs were developed, evaluated and compared. Both methods showed a good precision and detection limits, with no signs of inaccuracy and could be applied to biochemical autoanalyzers usually found in clinical laboratories using only minimal amounts of serum. Additionally the behaviour of Cp in experimental and clinical situations was studied showing an increase of around two fold after turpentine administration and significantly higher values in cases of pigs with inflammatory conditions when compared with healthy pigs.
铜蓝蛋白（Cp）测定可为动物健康状况提供客观指标，并可作为动物健康和福利的标志物（Skinner，2001年）（Skinner，J.G.，2001年。特别报告。急性期蛋白的国际标准化。兽医临床病理学30，2 - 7），但据报道，仅有人工方法用于测定猪的Cp浓度（McCosker，1961年；Toussaint等人，1995年；Eckersall等人，1996年）（McCosker，P.J.，1961年。哺乳动物血浆中的对苯二胺氧化酶活性和铜水平。自然。190，887 - 889；Toussaint，M.J.M.，Van Ederen，A.M.，Gruys，E.，1995年。临床病理学在动物健康评估、动物生产和肉类检验中的意义。比较血液学国际5，149 - 157；Eckersall，P.D.，Saini，P.K.，McComb，C.，1996年。猪中酸溶性糖蛋白、α(1)-酸性糖蛋白、铜蓝蛋白、触珠蛋白和C反应蛋白的急性期反应。兽医免疫学与免疫病理学51，377 - 385）。在本研究中，开发、评估并比较了两种基于使用两种不同底物测定猪血清铜蓝蛋白的自动化方法。两种方法均显示出良好的精密度和检测限，没有不准确的迹象，并且仅使用少量血清即可应用于临床实验室中常见的生化自动分析仪。此外，还研究了Cp在实验和临床情况下的表现，结果显示注射松节油后Cp增加约两倍，与健康猪相比，患有炎症的猪的Cp值显著更高。
