Plotnikova J M, Rahme L G, Ausubel F M
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Plant Physiol. 2000 Dec;124(4):1766-74. doi: 10.1104/pp.124.4.1766.
The human opportunistic pathogen Pseudomonas aeruginosa strain PA14 is a multihost pathogen that can infect Arabidopsis. We found that PA14 pathogenesis in Arabidopsis involves the following steps: attachment to the leaf surface, congregation of bacteria at and invasion through stomata or wounds, colonization of intercellular spaces, and concomitant disruption of plant cell wall and membrane structures, basipetal movement along the vascular parenchyma, and maceration and rotting of the petiole and central bud. Distinctive features of P. aeruginosa pathogenesis are that the surface of mesophyll cell walls adopt an unusual convoluted or undulated appearance, that PA14 cells orient themselves perpendicularly to the outer surface of mesophyll cell walls, and that PA14 cells make circular perforations, approximately equal to the diameter of P. aeruginosa, in mesophyll cell walls. Taken together, our data show that P. aeruginosa strain PA14 is a facultative pathogen of Arabidopsis that is capable of causing local and systemic infection, which can result in the death of the infected plant.
人类机会致病菌铜绿假单胞菌PA14菌株是一种可感染拟南芥的多宿主病原体。我们发现PA14在拟南芥中的致病过程涉及以下步骤：附着于叶片表面，在气孔或伤口处聚集并通过气孔或伤口侵入，在细胞间隙定殖，并伴随植物细胞壁和膜结构的破坏，沿维管束薄壁组织向基部移动，以及叶柄和顶芽的浸软和腐烂。铜绿假单胞菌致病的独特特征是叶肉细胞壁表面呈现出异常的卷曲或起伏外观，PA14细胞垂直于叶肉细胞壁的外表面排列，并且PA14细胞在叶肉细胞壁上形成直径约等于铜绿假单胞菌直径的圆形穿孔。综合来看，我们的数据表明铜绿假单胞菌PA14菌株是拟南芥的兼性病原体，能够引起局部和全身感染，这可能导致受感染植物死亡。
