Rahme L G, Stevens E J, Wolfort S F, Shao J, Tompkins R G, Ausubel F M
Department of Genetics, Harvard Medical School, Boston, MA 02114, USA.
Science. 1995 Jun 30;268(5219):1899-902. doi: 10.1126/science.7604262.
A Pseudomonas aeruginosa strain (UCBPP-PA14) is infectious both in an Arabidopsis thaliana leaf infiltration model and in a mouse full-thickness skin burn model. UCBPP-PA14 exhibits ecotype specificity for Arabidopsis, causing a range of symptoms from none to severe in four different ecotypes. In the mouse model, UCBPP-PA14 is as lethal as other well-studied P. aeruginosa strains. Mutations in the UCBPP-PA14 toxA, plcS, and gacA genes resulted in a significant reduction in pathogenicity in both hosts, indicating that these genes encode virulence factors required for the full expression of pathogenicity in both plants and animals.
一株铜绿假单胞菌（UCBPP - PA14）在拟南芥叶片浸润模型和小鼠全层皮肤烧伤模型中均具有传染性。UCBPP - PA14对拟南芥表现出生态型特异性，在四种不同生态型中引发从无到严重的一系列症状。在小鼠模型中，UCBPP - PA14与其他经过充分研究的铜绿假单胞菌菌株一样具有致死性。UCBPP - PA14的toxA、plcS和gacA基因发生突变后，在两种宿主中的致病性均显著降低，这表明这些基因编码植物和动物中致病性充分表达所需的毒力因子。
