Krymskiĭ L D, Arshinova M N
Biull Eksp Biol Med. 1975 Jan;79(1):90-3.
Not only fixation of material, but also conditions of studying it in the raster electron microscope influence the quality of the image. In case the carbon or the heavy metal layer is thin there is danger during rapid scanning of charging the sample by the electron beam, the quality of the image thus becoming worse. It is best to study the biological samples at the two nominals of the accelerating voltage (for example, 10 and 4 kV); this permits to avoid mistakes in interpretation of the results. Neither the thickness of the carbon or the heavy metal layer not the different nominals of the accelerating voltage could influence the value of the information obtained if analysis with consideration to the physical basis of the image formation and experimental conditions were conducted in each individual case. The archives material fixated in common formalin can be used for the study in the raster electron microscope.
不仅材料的固定，而且在光栅电子显微镜中研究材料的条件都会影响图像质量。如果碳层或重金属层很薄，在快速扫描期间存在电子束使样品带电的风险，图像质量因此会变差。最好在两个加速电压标称值下研究生物样品（例如，10 kV 和 4 kV）；这有助于避免结果解释中的错误。如果在每个具体情况下都结合图像形成的物理基础和实验条件进行分析，那么碳层或重金属层的厚度以及加速电压的不同标称值都不会影响所获得信息的价值。保存在普通福尔马林中的存档材料可用于在光栅电子显微镜中进行研究。
