Gross W, Heinrich D, Walker P, Mersch-Baumert K, Mueller-Eckhardt C
Blut. 1975 Feb;30(2):101-6. doi: 10.1007/BF01633964.
3 human antisera exhibiting non HL-A specific antibody were studied in micro-complement fixation with platelets and micro-lymphocytotoxicity. Screening studies as well as absorption and elution studies with platelets, lymphocytes and erythrocytes revealed an antibody activity associated with blood group A-1. Within blood group A-1, a subpopulation of A-1 donor cells- provisionally called "weak A-1" - was defined, which had a minor ability for complement-fixing and lymphocytotoxic A-1-antibody absorption. Contrary to HL-A specific antibodies, acid eluates from platelets of these 3 sera were always negative whereas heat and ether eluates gave positive results. From neutralization studies and studies on the hemolytic activity against A-1 erythrocytes it was concluded, that the A-1-specific antibodies probably are immune antibodies belonging to the IgG class of immunoglobulins.
对3种表现出非HL - A特异性抗体的人抗血清进行了血小板微量补体结合试验和微量淋巴细胞毒性试验研究。用血小板、淋巴细胞和红细胞进行的筛选研究以及吸收和洗脱研究显示，一种抗体活性与A - 1血型相关。在A - 1血型中，定义了一个A - 1供体细胞亚群——暂称为“弱A - 1”，其补体结合和淋巴细胞毒性A - 1抗体吸收能力较弱。与HL - A特异性抗体相反，这3种血清的血小板酸洗脱物始终为阴性，而热洗脱物和乙醚洗脱物则呈阳性结果。通过中和研究以及针对A - 1红细胞的溶血活性研究得出结论，A - 1特异性抗体可能是属于免疫球蛋白IgG类的免疫抗体。
