Cui Q, Harvey A R
Department of Anatomy and Human Biology, The University of Western Australia, Perth, Australia.
Neuroreport. 2000 Nov 27;11(17):3921-4. doi: 10.1097/00001756-200011270-00062.
Using the TUNEL method, we examined the effect of intraocular NT-4/5 injections on cell death in ganglion and non-ganglion cell layers in 5-day-old rat retinas. NT-4/5 reduced the level of naturally occurring cell death in all retinal layers. Twenty-four hours after superior colliculus (SC) lesions there was a significant increase in the density of TUNEL+ profiles in the RGC layer (6.43/mm2 in normal vs (8.89/mm2 after lesions) which was ameliorated by intraocular NT-4/5 injections (8.79/mm2). Surprisingly, after SC ablation a significant increase in TUNEL+ profiles was also seen in non-ganglion cell layers (52.25/mm2 in normal vs 89.35/mm2 after lesions), mostly in the developing inner nuclear layer. Death in non-ganglion cell layers was also significantly reduced (43.09/mm2) after NT-4/5 eye injections.
我们采用TUNEL法，研究了眼内注射NT - 4/5对5日龄大鼠视网膜神经节细胞层和非神经节细胞层细胞死亡的影响。NT - 4/5降低了所有视网膜层自然发生的细胞死亡水平。上丘（SC）损伤24小时后，视网膜神经节细胞（RGC）层TUNEL阳性细胞密度显著增加（正常时为6.43/mm²，损伤后为8.89/mm²），而眼内注射NT - 4/5可改善这种情况（8.79/mm²）。令人惊讶的是，SC切除后，非神经节细胞层的TUNEL阳性细胞也显著增加（正常时为52.25/mm²，损伤后为89.35/mm²），主要发生在发育中的内核层。眼内注射NT - 4/5后，非神经节细胞层的细胞死亡也显著减少（43.09/mm²）。
