Cui Q, Harvey A R
Department of Anatomy and Human Biology, University of Western Australia, Nedlands, Perth.
Neuroreport. 1994 Oct 3;5(15):1882-4. doi: 10.1097/00001756-199410000-00010.
The retrograde nucleophilic tracer diamidino yellow (DY) was injected into the left superior colliculi of 2-day-old rats. Two days later, right eyes were injected with either neurotrophin NT-4/5, cycloheximide (CHX), MK-801 and DNQX (glutamate receptor antagonists), or saline. Almost all rats were perfused 6-7 h later and the numbers of normal and pyknotic DY-labelled retinal ganglion cells (RGCs) were determined from retinal whole mounts. In controls (no eye injection) the proportion of pyknotic RGCs was 1.04%. This level of naturally occurring death was significantly reduced after injection of NT-4/5 (0.34%); normal RGC density was also higher in this group. RGC pyknosis was increased after saline (1.21%), MK-801/DNQX (1.22%) or CHX (1.48%) injections but only in the latter case was the increase significantly greater than control.
将逆行性亲核示踪剂双脒基黄（DY）注入2日龄大鼠的左视上丘。两天后，右眼注射神经营养因子NT-4/5、放线菌酮（CHX）、MK-801和DNQX（谷氨酸受体拮抗剂）或生理盐水。几乎所有大鼠在6-7小时后被灌注，并从视网膜整装片中确定正常和固缩的DY标记视网膜神经节细胞（RGC）的数量。在对照组（未注射眼睛）中，固缩RGC的比例为1.04%。注射NT-4/5后，这种自然发生的死亡水平显著降低（0.34%）；该组中正常RGC密度也更高。注射生理盐水（1.21%）、MK-801/DNQX（1.22%）或CHX（1.48%）后，RGC固缩增加，但仅在最后一种情况下，增加显著大于对照组。
