对从丹麦一家火鸡加工厂、火鸡产品以及人类李斯特菌病病例中分离出的单核细胞增生李斯特菌进行的比较研究。
Comparative investigations of Listeria monocytogenes isolated from a turkey processing plant, turkey products, and from human cases of listeriosis in Denmark.
作者信息
Ojeniyi B, Christensen J, Bisgaard M
机构信息
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Frederiksberg C, Copenhagen, Denmark.
出版信息
Epidemiol Infect. 2000 Oct;125(2):303-8. doi: 10.1017/s0950268899004483.
Listeria monocytogenes was isolated from critical control points in a Danish turkey processing plant, from turkey products and from cases of human listeriosis. During processing in the plant the prevalence of L. monocytogenes ranged from 25.9 to 41.4%. Cleaning and disinfection decreased the prevalence to 6.4%. Isolates of L. monocytogenes were characterized by pulsed-field gel electrophoresis (PFGE) using restriction endonuclease ApaI. Identical DNA types were obtained from turkey products and the processing line even after cleaning and disinfection. Two identical DNA types were demonstrated among isolates from turkey products and human cases of listeriosis. The prevalence of L. monocytogenes in turkey products ranged from 7.3 to 17.4% for ready-to-eat products and raw products, respectively. Since none of the 27 flocks examined before slaughter sampled positive for L. monocytogenes and the prevalence increased during processing, the potential risk from turkey meat was apparently due to factory hygiene rather than intrinsic contamination of the turkeys.
从丹麦一家火鸡加工厂的关键控制点、火鸡产品以及人类李斯特菌病病例中分离出了单核细胞增生李斯特菌。在工厂加工过程中，单核细胞增生李斯特菌的检出率在25.9%至41.4%之间。清洁和消毒后，检出率降至6.4%。使用限制性内切酶ApaI通过脉冲场凝胶电泳（PFGE）对单核细胞增生李斯特菌分离株进行了特征分析。即使在清洁和消毒后，从火鸡产品和加工生产线中仍获得了相同的DNA类型。在火鸡产品分离株和人类李斯特菌病病例中发现了两种相同的DNA类型。即食产品和生鲜产品中单核细胞增生李斯特菌的检出率分别为7.3%至17.4%。由于在屠宰前检查的27群火鸡中均未检测到单核细胞增生李斯特菌呈阳性，且在加工过程中检出率有所上升，因此火鸡肉的潜在风险显然源于工厂卫生状况，而非火鸡本身的内在污染。
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