Hoelzle L E, Steinhausen G, Wittenbrink M M
Institute for Medical Microbiology, Infectious and Epidemic Diseases, Ludwig-Maximilians-University, Munich, Germany.
Epidemiol Infect. 2000 Oct;125(2):427-39. doi: 10.1017/s0950268899004446.
Lung and intestine of 49 pigs with respiratory diseases and endocervical swabs from 205 sows with reproductive disorders were investigated for chlamydial infection by polymerase chain reaction. PCR primers targeted DNA sequences on the chlamydial omp1 or omp2 genes. PCR amplicons were generated from 49.0% of pigs with respiratory disease, from 60.0% of sows with reproductive disorders, from 24.5% of respiratory healthy controls, but from no endocervical swabs from fertile sows. By DNA hybridization, a high prevalence of mixed infections with Chlamydophila abortus and Chlamydia suis in the porcine lung and intestine was found and confirmed by RFLP and nucleotide analysis. Of the omp1-PCR amplicons from endocervical swabs 81.3% were identified as Chlamydophila abortus, indicating an association of this chlamydial species with reproductive disorders in sows. Nucleotide sequence analysis of omp1-amplicons identified as deriving from Chlamydia suis shared a maximum of 82.7% homology with the reference strain S45.
采用聚合酶链反应对49头患有呼吸道疾病的猪的肺和肠道以及205头患有生殖障碍的母猪的子宫颈拭子进行衣原体感染调查。聚合酶链反应引物靶向衣原体omp1或omp2基因上的DNA序列。在患有呼吸道疾病的猪中，49.0%产生了聚合酶链反应扩增子；在患有生殖障碍的母猪中，60.0%产生了扩增子；在呼吸道健康对照猪中，24.5%产生了扩增子；而在可育母猪的子宫颈拭子中未产生扩增子。通过DNA杂交，发现在猪的肺和肠道中流产嗜衣原体和猪衣原体混合感染的发生率很高，并通过限制性片段长度多态性和核苷酸分析得到证实。在来自子宫颈拭子的omp1 -聚合酶链反应扩增子中，81.3%被鉴定为流产嗜衣原体，表明该衣原体种类与母猪的生殖障碍有关。被鉴定为源自猪衣原体的omp1 -扩增子的核苷酸序列分析显示，与参考菌株S45的同源性最高为82.7%。
