核因子1（NF1）影响人类RNA聚合酶III的精确终止和多轮转录。
Nuclear factor 1 (NF1) affects accurate termination and multiple-round transcription by human RNA polymerase III.
作者信息
Wang Z, Bai L, Hsieh Y J, Roeder R G
机构信息
The Laboratory of Biochemistry and Molecular Biology,The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
出版信息
EMBO J. 2000 Dec 15;19(24):6823-32. doi: 10.1093/emboj/19.24.6823.
Abstract
We have shown previously that the TFIIIC1/TFIIIC1' fraction interacts specifically with the VA1 terminator regions to affect both termination and initiation/reinitiation of transcription by human RNA polymerase III. Here, we further purified the VA1 terminator-binding factor to apparent homogeneity and found, by peptide sequence analysis, that it belongs to the NF1 protein family. NF1 interacts specifically with the NF1-binding sites within the terminator regions of the VA1 gene and with two subunits (TFIIIC220 and TFIIIC110) of human TFIIIC2. Immunodepletion with anti-NF1 antibodies dramatically decreases transcription from the VA1 template in nuclear extract, and mutation at the NF1-binding site in the terminator region of the VA1 gene selectively affects multiple-round transcription (reinitiation of transcription) and termination. In addition, NF1 acts in conjunction with TFIIIC to promote accurate termination by RNA polymerase III on a C-tailed VA1 template.
摘要
我们之前已经表明，TFIIIC1/TFIIIC1'组分与VA1终止子区域特异性相互作用，以影响人RNA聚合酶III的转录终止以及起始/重新起始。在此，我们进一步将VA1终止子结合因子纯化至表观均一性，并通过肽序列分析发现，它属于NF1蛋白家族。NF1与VA1基因终止子区域内的NF1结合位点以及人TFIIIC2的两个亚基（TFIIIC220和TFIIIC110）特异性相互作用。用抗NF1抗体进行免疫去除可显著降低核提取物中VA1模板的转录，并且VA1基因终止子区域中NF1结合位点的突变选择性地影响多轮转录（转录重新起始）和终止。此外，NF1与TFIIIC协同作用，以促进RNA聚合酶III在C尾VA1模板上的精确终止。
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