Ganesh K, Suryanarayana V, Raghavan R, Gowda S
Indian Veterinary Research Institute, Hebbal, 560024, Karnataka, Bangalore, India.
Vet Microbiol. 2001 Jan 5;78(1):1-11. doi: 10.1016/s0378-1135(00)00288-1.
In order to study the serotypic variations in hydropericardium hepatitis syndrome (HHS) causing virus, the DNA was extracted from the purified virus, a 0.7 kb variable region of hexon gene encoding L1 and part of P1 amplified and sequenced. Both nucleotide and derived amino acid sequences, corresponding to the variable region, were compared with the published fowl adenovirus sequences (FAV serotypes 10, 1 and 8). As expected the 0.7 kb sequence showed single open reading frame (ORF). There was a nucleotide sequence variation of 8.2, 28.1 and 40.3%, respectively, with FAV serotypes 10, 1 and 8. The dendrogram constructed with the nucleotide sequences showed that HHS virus and FAV10 are closer to each other and are away to FAV1 and FAV8. However, the derived amino acid sequence showed variations as high as 28.8, 38 and 45.1% with FAV serotypes 10, 1 and 8, respectively. Such high degree variation has been found due to the shift in the reading frame caused by deletions indicating that the FAV4 associated with HHS is unique and different from FAV10. To the best of our knowledge this is the first report on nucleotide sequence analysis of hexon gene fragment of FAV4 associated with HHS.
为研究引起心包积水肝炎综合征（HHS）的病毒的血清型变异情况，从纯化病毒中提取DNA，扩增并测序编码L1的六邻体基因0.7 kb可变区及部分P1。将对应可变区的核苷酸序列和推导的氨基酸序列与已发表的禽腺病毒序列（FAV血清型10、1和8）进行比较。正如预期的那样，0.7 kb序列显示出单一开放阅读框（ORF）。与FAV血清型10、1和8相比，核苷酸序列变异分别为8.2%、28.1%和40.3%。根据核苷酸序列构建的系统发育树表明，HHS病毒与FAV10彼此更接近，而与FAV1和FAV8距离较远。然而，推导的氨基酸序列与FAV血清型10、1和8相比，变异分别高达28.8%、38%和45.1%。由于缺失导致阅读框移位，发现了如此高的变异程度，这表明与HHS相关的FAV4是独特的，不同于FAV10。据我们所知，这是关于与HHS相关的FAV4六邻体基因片段核苷酸序列分析的首次报告。
