来自印度的禽腺病毒4分离株六邻体基因L1环可变区的核苷酸序列分析

Nucleotide sequence analysis of the L1 loop variable region of hexon gene of fowl adenovirus 4 isolates from India.

作者信息

Parthiban M, Manoharan S, Roy P, Chandran N D J, Aruni A W, Koteeswaran A

机构信息

Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 051, India.

出版信息

Acta Virol. 2005;49(1):65-8.

PMID:15929401

Abstract

Three fowl adenovirus 4 (FAV4) isolates from chicken and one from quail, all from Tamil Nadu, India were analyzed. The L1 loop variable region of hexon gene of these isolates was amplified by PCR and sequenced. The nucleotide sequences (442 bp) and deduced amino acid sequences of the four isolates were compared with those of other isolates of FAV4. The nucleotide sequences of the four isolates had a 98% homology with other Indian isolates and a 96% homology with Belgian and Russian isolates. The amino acid sequences of the four Indian isolates had a more than 98% homology with other Indian isolates and a more than 92% homology with Belgian and Russian isolates. Hence, the variable of L1 loop region of hexon gene was found to be highly homologous in all the FAV4 isolates tested both at nucleotide and amino acid level.

摘要

对来自印度泰米尔纳德邦的3株鸡源和1株鹌鹑源禽腺病毒4型（FAV4）分离株进行了分析。通过PCR扩增并测序这些分离株六邻体基因的L1环可变区。将这4株分离株的核苷酸序列（442 bp）和推导的氨基酸序列与其他FAV4分离株进行比较。这4株分离株的核苷酸序列与其他印度分离株有98%的同源性，与比利时和俄罗斯分离株有96%的同源性。这4株印度分离株的氨基酸序列与其他印度分离株有超过98%的同源性，与比利时和俄罗斯分离株有超过92%的同源性。因此，发现在所有测试的FAV4分离株中，六邻体基因L1环区域在核苷酸和氨基酸水平上都具有高度同源性。