Wainwright M
Photochemotherapy Group, Department of Biological Sciences, University of Central Lancashire, Preston PR1 2HE, UK.
Int J Antimicrob Agents. 2000 Dec;16(4):381-94. doi: 10.1016/s0924-8579(00)00207-7.
Photodynamic antimicrobial agents based on the well-established phenothiazinium biological stain methylene blue offer a simple method for the inactivation or destruction of pathogens contained in donated blood and blood products. The technique is currently concentrated on viruses and the disinfective procedure can be carried out in blood bags using basic low-power light sources. Pathogens of the bacterial, yeast and protozoal classes are also susceptible to phenothiaziniums. The photoantimicrobial mode of action is usually via oxidative damage to cellular components, either due to redox reactions between the agent and a biomolecular target or by the action of reactive oxygen species generated in situ by photodynamic action. The targeting of various microbial species is discussed in relation to the physicochemical make-up of the photosensitizers, and future directions are suggested.
基于成熟的吩噻嗪类生物染色剂亚甲蓝的光动力抗菌剂为灭活或破坏捐献血液和血液制品中所含病原体提供了一种简单方法。该技术目前主要针对病毒，消毒过程可在血袋中使用基本的低功率光源进行。细菌、酵母和原生动物类别的病原体也对吩噻嗪类敏感。光抗菌作用模式通常是通过对细胞成分的氧化损伤，这要么是由于该试剂与生物分子靶点之间的氧化还原反应，要么是由于光动力作用原位产生的活性氧的作用。结合光敏剂的物理化学组成讨论了对各种微生物物种的靶向作用，并提出了未来的发展方向。
