牛乳头瘤病毒1型E5和E7蛋白的协同转化与共表达
Cooperative transformation and coexpression of bovine papillomavirus type 1 E5 and E7 proteins.
作者信息
Bohl J, Hull B, Vande Pol S B
机构信息
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106, USA.
出版信息
J Virol. 2001 Jan;75(1):513-21. doi: 10.1128/JVI.75.1.513-521.2001.
Abstract
Productively infected bovine fibropapillomas were examined for bovine papillomavirus type 1 (BPV-1) E7 localization. BPV-1 E7 was observed in the cytoplasm of basal and lower spinous epithelial cells, coexpressed in the cytoplasm of basal cells with the E5 oncoprotein. E7 was also observed in nucleoli throughout the basal and spinous layers but not in the granular cell layer. Ectopic expression of E7 in cultured epithelial cells gave rise to localization similar to that seen in productive fibropapillomas, with cytoplasmic and nucleolar expression observed. Consistent with the coexpression of E7 and E5 in basal keratinocytes, BPV-1 E7 cooperated with E5 as well as E6 in an anchorage independence transformation assay. While E5 is expressed in both basal and superficial differentiating keratinocytes, BPV-1 E7 is only observed in basal and lower spinous epithelial cells. Therefore, BPV-1 E7 may serve to modulate the cellular response of basal epithelial cells to E5 expression.
摘要
对产生病毒的感染性牛纤维乳头瘤进行了1型牛乳头瘤病毒（BPV-1）E7定位检查。在基底和棘层下部上皮细胞的细胞质中观察到BPV-1 E7，在基底细胞的细胞质中与E5癌蛋白共表达。在整个基底和棘层的核仁中也观察到E7，但在颗粒细胞层中未观察到。E7在培养的上皮细胞中的异位表达产生了与在产生病毒的纤维乳头瘤中所见相似的定位，观察到细胞质和核仁表达。与E7和E5在基底角质形成细胞中的共表达一致，在锚定非依赖性转化试验中，BPV-1 E7与E5以及E6协同作用。虽然E5在基底和表面分化的角质形成细胞中均有表达，但BPV-1 E7仅在基底和棘层下部上皮细胞中观察到。因此，BPV-1 E7可能有助于调节基底上皮细胞对E5表达的细胞反应。
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