囊性纤维化患者中嗜中性粒细胞向白细胞介素-8的定向迁移增加：一项关于红霉素作用的研究。
Directed neutrophil migration to IL-8 is increased in cystic fibrosis: a study of the effect of erythromycin.
作者信息
Brennan S, Cooper D, Sly P D
机构信息
Division of Clinical Sciences, TVW Telethon Institute for Child Health Research, Subiaco, Western Australia.
出版信息
Thorax. 2001 Jan;56(1):62-4. doi: 10.1136/thorax.56.1.62.
Abstract
BACKGROUND
The aim of this study was to compare neutrophil migration in cystic fibrosis (CF) and non-CF populations and to investigate the effect of erythromycin on directed migration of neutrophils (PMNs) in CF.
METHODS
PMNs were isolated and their migratory capacity in response to interleukin-8 (IL-8) or f-Met-Leu-Phe (fMLP) in the presence or absence of erythromycin (1-100 microg/ml) was assessed.
RESULTS
CF derived PMNs showed significantly increased migration to IL-8 but not to fMLP compared with non-CF PMNs. Erythromycin had no significant effect on migration responses to IL-8 and in vitro exposure of PMNs to erythromycin had no effect.
CONCLUSIONS
CF derived PMNs show higher migratory responsiveness to IL-8 but not to fMLP, suggesting that CF PMNs may be "primed" to IL-8 which is significantly increased in CF serum compared with non-CF serum. Treatment with erythromycin had no significant effect on PMN migration in vitro.
摘要
背景
本研究旨在比较囊性纤维化（CF）患者和非CF人群中中性粒细胞的迁移情况，并研究红霉素对CF患者中性粒细胞（PMN）定向迁移的影响。
方法
分离PMN，评估在有或无红霉素（1-100微克/毫升）存在的情况下，它们对白细胞介素-8（IL-8）或N-甲酰甲硫氨酸-亮氨酸-苯丙氨酸（fMLP）的迁移能力。
结果
与非CF的PMN相比，CF来源的PMN对IL-8的迁移显著增加，但对fMLP的迁移无明显增加。红霉素对PMN对IL-8的迁移反应无显著影响，并且PMN在体外暴露于红霉素也无影响。
结论
CF来源的PMN对IL-8显示出更高的迁移反应性，但对fMLP无此反应，这表明CF的PMN可能对IL-8处于“致敏”状态，与非CF血清相比，CF血清中的IL-8显著增加。红霉素治疗对体外PMN迁移无显著影响。
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