Rossi E C, Lousi G
J Lab Clin Med. 1975 Feb;85(2):300-6.
The variable responsiveness of platelet-rich plasma (PRP) to epinephrine and collagen was studied. These studies revealed that as PRP is allowed to stand at room temperature it becomes progressively more responsive to the aggregating effects of epinephrine and collagen. Samples of PRP obtained from 10 normal subjects showed only 8 plus or minus 8 per cent aggregation when tested after 5 minutes with "near threshold" concentrations of epinephrine. After 45 minutes the addition of the same concentrations of epinephrine to portions taken from the same samples of PRP yielded 72 plus or minus 12 per cent aggregation. Although similar results were obtained with "near threshold" concentrations of collagen, the variability of responsiveness to collagen could be easily overcome by increasing the collagen concentration. Such was not the case with epinephrine, and, a time-dependent acquisition of responsiveness was demonstrable over a broad range of epinephrine concentration. These findings suggest that variations in the amount of platelet aggregation observed with epinephrine may be a reflection of differences in the time factor involved in testing. This time factor should be taken into account before epinephrine-induced platelet aggregation curves are compared and defined as "normal" or "abnormal".
研究了富含血小板血浆（PRP）对肾上腺素和胶原蛋白的可变反应性。这些研究表明，随着PRP在室温下放置，它对肾上腺素和胶原蛋白的聚集作用的反应性逐渐增强。从10名正常受试者获得的PRP样本，在用“接近阈值”浓度的肾上腺素测试5分钟后，仅显示8±8%的聚集率。45分钟后，向取自相同PRP样本的部分中加入相同浓度的肾上腺素，聚集率为72±12%。尽管用“接近阈值”浓度的胶原蛋白也得到了类似结果，但通过增加胶原蛋白浓度可以很容易地克服对胶原蛋白反应性的变异性。肾上腺素并非如此，在广泛的肾上腺素浓度范围内，可证明存在时间依赖性的反应性获得。这些发现表明，用肾上腺素观察到的血小板聚集量的变化可能反映了测试中所涉及时间因素的差异。在比较肾上腺素诱导的血小板聚集曲线并将其定义为“正常”或“异常”之前，应考虑这个时间因素。
