植物拟南芥5号染色体的测序与分析。

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.

作者信息

Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See L H, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie W R, Wilson R K, Murphy G, Bancroft I, Volckaert G, Wambutt R, Düsterhöft A, Stiekema W, Pohl T, Entian K D, Terryn N, Hartley N, Bent E, Johnson S, Langham S A, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst R K, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes H W, Bevan M, Fransz P

机构信息

Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.

出版信息

Nature. 2000 Dec 14;408(6814):823-6. doi: 10.1038/35048507.

DOI:10.1038/35048507

PMID:11130714

Abstract

The genome of the model plant Arabidopsis thaliana has been sequenced by an international collaboration, The Arabidopsis Genome Initiative. Here we report the complete sequence of chromosome 5. This chromosome is 26 megabases long; it is the second largest Arabidopsis chromosome and represents 21% of the sequenced regions of the genome. The sequence of chromosomes 2 and 4 have been reported previously and that of chromosomes 1 and 3, together with an analysis of the complete genome sequence, are reported in this issue. Analysis of the sequence of chromosome 5 yields further insights into centromere structure and the sequence determinants of heterochromatin condensation. The 5,874 genes encoded on chromosome 5 reveal several new functions in plants, and the patterns of gene organization provide insights into the mechanisms and extent of genome evolution in plants.

摘要

模式植物拟南芥的基因组已由国际合作项目“拟南芥基因组计划”完成测序。在此，我们报告第5号染色体的完整序列。这条染色体长26兆碱基对；它是拟南芥第二大染色体，占基因组测序区域的21%。第2号和第4号染色体的序列此前已公布，第1号和第3号染色体的序列以及完整基因组序列分析在本期发表。对第5号染色体序列的分析进一步揭示了着丝粒结构以及异染色质浓缩的序列决定因素。第5号染色体上编码的5874个基因揭示了植物中的一些新功能，基因组织模式为了解植物基因组进化的机制和程度提供了线索。