对拟南芥4号染色体上1.9兆碱基连续序列的分析。

Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana.

作者信息

Bevan M, Bancroft I, Bent E, Love K, Goodman H, Dean C, Bergkamp R, Dirkse W, Van Staveren M, Stiekema W, Drost L, Ridley P, Hudson S A, Patel K, Murphy G, Piffanelli P, Wedler H, Wedler E, Wambutt R, Weitzenegger T, Pohl T M, Terryn N, Gielen J, Villarroel R, De Clerck R, Van Montagu M, Lecharny A, Auborg S, Gy I, Kreis M, Lao N, Kavanagh T, Hempel S, Kotter P, Entian K D, Rieger M, Schaeffer M, Funk B, Mueller-Auer S, Silvey M, James R, Montfort A, Pons A, Puigdomenech P, Douka A, Voukelatou E, Milioni D, Hatzopoulos P, Piravandi E, Obermaier B, Hilbert H, Düsterhöft A, Moores T, Jones J D, Eneva T, Palme K, Benes V, Rechman S, Ansorge W, Cooke R, Berger C, Delseny M, Voet M, Volckaert G, Mewes H W, Klosterman S, Schueller C, Chalwatzis N

机构信息

Department of Molecular Genetics, John Innes Centre, Colney, Norwich, UK.

出版信息

Nature. 1998 Jan 29;391(6666):485-8. doi: 10.1038/35140.

DOI:10.1038/35140

PMID:9461215

Abstract

The plant Arabidopsis thaliana (Arabidopsis) has become an important model species for the study of many aspects of plant biology. The relatively small size of the nuclear genome and the availability of extensive physical maps of the five chromosomes provide a feasible basis for initiating sequencing of the five chromosomes. The YAC (yeast artificial chromosome)-based physical map of chromosome 4 was used to construct a sequence-ready map of cosmid and BAC (bacterial artificial chromosome) clones covering a 1.9-megabase (Mb) contiguous region, and the sequence of this region is reported here. Analysis of the sequence revealed an average gene density of one gene every 4.8 kilobases (kb), and 54% of the predicted genes had significant similarity to known genes. Other interesting features were found, such as the sequence of a disease-resistance gene locus, the distribution of retroelements, the frequent occurrence of clustered gene families, and the sequence of several classes of genes not previously encountered in plants.

摘要

植物拟南芥已成为研究植物生物学诸多方面的重要模式物种。其相对较小的核基因组以及五条染色体广泛物理图谱的可得性，为启动五条染色体的测序提供了可行基础。基于酵母人工染色体（YAC）的4号染色体物理图谱被用于构建覆盖190万碱基对（Mb）连续区域的黏粒和细菌人工染色体（BAC）克隆的序列就绪图谱，本文报道了该区域的序列。对该序列的分析揭示，平均每4.8千碱基（kb）有一个基因，且54%的预测基因与已知基因有显著相似性。还发现了其他有趣的特征，如一个抗病基因位点的序列、反转录元件的分布、成簇基因家族的频繁出现，以及植物中此前未遇到的几类基因的序列。