Hansen L H, Ferrari B, Sørensen A H, Veal D, Sørensen S J
Department of General Microbiology, University of Copenhagen, DK-1307 Copenhagen K, Denmark.
Appl Environ Microbiol. 2001 Jan;67(1):239-44. doi: 10.1128/AEM.67.1.239-244.2001.
Combining the high specificity of bacterial biosensors and the resolution power of fluorescence-activated cell sorting (FACS) provided qualitative detection of oxytetracycline production by Streptomyces rimosus in soil microcosms. A plasmid containing a transcriptional fusion between the tetR-regulated P(tet) promoter from Tn10 and a FACS-optimized gfp gene was constructed. When harbored by Escherichia coli, this plasmid produces large amounts of green fluorescent protein (GFP) in the presence of tetracycline. This tetracycline biosensor was used to detect the production of oxytetracycline by S. rimosus introduced into sterile soil. The tetracycline-induced GFP-producing biosensors were detected by FACS analysis, enabling the detection of oxytetracycline encounters by single biosensor cells. This approach can be used to study interactions between antibiotic producers and their target organisms in soil.
将细菌生物传感器的高特异性与荧光激活细胞分选（FACS）的分辨能力相结合，实现了对土壤微观环境中龟裂链霉菌产生土霉素的定性检测。构建了一个质粒，该质粒包含来自Tn10的tetR调控的P(tet)启动子与一个FACS优化的gfp基因之间的转录融合。当大肠杆菌携带该质粒时，在四环素存在的情况下会产生大量绿色荧光蛋白（GFP）。这种四环素生物传感器用于检测引入无菌土壤中的龟裂链霉菌产生的土霉素。通过FACS分析检测四环素诱导产生GFP的生物传感器，从而能够检测单个生物传感器细胞遇到的土霉素。这种方法可用于研究土壤中抗生素生产者与其目标生物体之间的相互作用。
