Bégin M, Weber J
J Virol. 1975 Jan;15(1):1-7. doi: 10.1128/JVI.15.1.1-7.1975.
Temperature-sensitive mutants which replicate normally at 33 C but poorly at 39 C were isolated from nitrosoguanidine- or nitrous acid-mutagenized adenovirus 2 by (i) testing the cytopathic effect or inclusion body-forming capacity of random plaque isolates, or (ii) reduced plaque enlargement upon shifting from 33 to 39 C. Thirty-six mutants were isolated with 33 C/39 C plaque ratios varying from 20 to 10-5. Some of these mutants could be arranged into 13 groups by the complementation test. By means of recombination analysis a provisional linear genetic map was constructed.
从经亚硝基胍或亚硝酸诱变的腺病毒2中分离出温度敏感突变体,这些突变体在33℃时能正常复制,但在39℃时复制不佳,方法如下:(i) 检测随机噬斑分离株的细胞病变效应或包涵体形成能力,或 (ii) 从33℃转移到39℃后噬斑扩大减少。分离出36个突变体,其33℃/39℃噬斑比率在20到10^-5之间变化。其中一些突变体通过互补试验可分为13组。通过重组分析构建了一个临时的线性遗传图谱。