Grodzicker T, Anderson C, Sharp P A, Sambrook J
J Virol. 1974 Jun;13(6):1237-44. doi: 10.1128/JVI.13.6.1237-1244.1974.
Human adenovirus type 2 (Ad2) grows poorly in monkey cells, although this defect can be overcome by co-infection with simian virus 40 (SV40). The nondefective Ad2-SV40 hybrid virus, Ad2(+)ND1, replicates efficiently in both human and African green monkey kidney cells, presumably due to the insertion of SV40 sequences into the Ad2 DNA. Several mutants of Ad2(+)ND1 have been isolated that grow and plaque poorly in monkey cells, although they retain the ability to replicate and plaque efficiently in HeLa cells. One of these mutants (H39) has been examined in detail. Studies comparing the DNA of the mutant with Ad2(+)ND1 either by the cleavage patterns produced by Escherichia coli R.RI restriction endonuclease digestion or by heteroduplexing reveal no differences. The pattern of protein synthesis of Ad2(+)ND1 and H39 in monkey cells is quite different with the mutant resembling Ad2, which is defective in the synthesis of late proteins. However, in human cells, the proteins synthesized by H39 and the parent Ad2(+)ND1 are very similar. The production of SV40 U antigen in H39-infected cells is different from that in Ad2(+)ND1-infected cells. Finally, the growth of H39 in monkey cells can be complemented by SV40.
人腺病毒2型（Ad2）在猴细胞中生长不佳，不过这种缺陷可通过与猿猴病毒40（SV40）共同感染来克服。无缺陷的Ad2 - SV40杂交病毒Ad2(+)ND1在人和非洲绿猴肾细胞中均能高效复制，推测这是由于SV40序列插入到了Ad2 DNA中。已分离出几种Ad2(+)ND1的突变体，它们在猴细胞中生长和形成噬斑的能力较差，尽管它们在HeLa细胞中仍保留高效复制和形成噬斑的能力。其中一个突变体（H39）已被详细研究。通过大肠杆菌R.RI限制性内切酶消化产生的切割模式或通过异源双链分析，比较突变体与Ad2(+)ND1的DNA，未发现差异。Ad2(+)ND1和H39在猴细胞中的蛋白质合成模式差异很大，该突变体类似于Ad2，在晚期蛋白质合成方面存在缺陷。然而，在人细胞中，H39和亲本Ad2(+)ND1合成的蛋白质非常相似。H39感染细胞中SV40 U抗原的产生与Ad2(+)ND1感染细胞中的不同。最后，SV40可互补H39在猴细胞中的生长。
