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通过对血清型间重组体进行限制性内切酶分析对2型腺病毒温度敏感突变进行物理图谱分析。

Physical mapping of adenovirus type 2 temperature-sensitive mutations by restriction endonuclease analysis of interserotypic recombinants.

作者信息

D'Halluin J C, Cousin C, Boulanger P

出版信息

J Virol. 1982 Feb;41(2):401-13. doi: 10.1128/JVI.41.2.401-413.1982.

DOI:10.1128/JVI.41.2.401-413.1982
PMID:6281456
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256770/
Abstract

The genome structures of about 100 interserotypic ts recombinants produced in crosses between human adenovirus type 2 (H2) and 5 (H5) temperature-sensitive mutants were analyzed by cleavage with restriction endonucleases to determine the map coordinates of the following temperature-sensitive mutants: penton base plus fiber-defective H2 ts103, -104, and -136, assembly-defective H2 ts112, fiber-defective H2 ts125, hexon-defective H2 ts118 and -121, and DNA-negative H2 ts111. H5 ts1 (100 K defective), H5 ts36 (DNA negative), H5 ts125 (mutated in the early 72,000-dalton protein), H5 ts22 (fiber defective), H5 ts58 (IIIa defective), and H5 ts18 and -19 were used as one of the parents. The physical locations of the H2 temperature-sensitive mutations thus defined are discussed in relation to the genetic map, the biological function altered, and the positions of the structural genes on the genome.

摘要

通过用限制性内切酶切割，分析了在人2型腺病毒（H2）和5型腺病毒（H5）温度敏感突变体杂交中产生的约100种血清型间ts重组体的基因组结构，以确定以下温度敏感突变体的图谱坐标：五邻体基座加纤维缺陷型H2 ts103、-104和-136，装配缺陷型H2 ts112，纤维缺陷型H2 ts125，六邻体缺陷型H2 ts118和-121，以及DNA阴性型H2 ts111。H5 ts1（100K缺陷型）、H5 ts36（DNA阴性型）、H5 ts125（在早期72000道尔顿蛋白中发生突变）、H5 ts22（纤维缺陷型）、H5 ts58（IIIa缺陷型）以及H5 ts18和-19用作亲本之一。本文结合遗传图谱、改变的生物学功能以及基因组上结构基因的位置，讨论了如此确定的H2温度敏感突变的物理位置。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e69f/256770/d957f36f2efb/jvirol00161-0066-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e69f/256770/c34bf40ee529/jvirol00161-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e69f/256770/d957f36f2efb/jvirol00161-0066-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e69f/256770/c34bf40ee529/jvirol00161-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e69f/256770/d957f36f2efb/jvirol00161-0066-b.jpg

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本文引用的文献

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