嗜热栖热菌磷酸果糖激酶：两种独特酶的表达与特性
Thermotoga maritima phosphofructokinases: expression and characterization of two unique enzymes.
作者信息
Ding Y R, Ronimus R S, Morgan H W
机构信息
Thermophile Research Unit, Department of Biological Sciences, The University of Waikato, Hamilton, New Zealand.
出版信息
J Bacteriol. 2001 Jan;183(2):791-4. doi: 10.1128/JB.183.2.791-794.2001.
Abstract
A pyrophosphate-dependent phosphofructokinase (PP(i)-PFK) and an ATP-dependent phosphofructokinase (ATP-PFK) from Thermotoga maritima have been cloned and characterized. The PP(i)-PFK is unique in that the K(m) and V(max) values indicate that polyphosphate is the preferred substrate over pyrophosphate; the enzyme in reality is a polyphosphate-dependent PFK. The ATP-PFK was not significantly affected by common allosteric effectors (e.g., phosphoenolpyruvate) but was strongly inhibited by PP(i) and polyphosphate. The results suggest that the control of the Embden-Meyerhof pathway in this organism is likely to be modulated by pyrophosphate and/or polyphosphate.
摘要
嗜热栖热菌的焦磷酸依赖性磷酸果糖激酶（PP(i)-PFK）和ATP依赖性磷酸果糖激酶（ATP-PFK）已被克隆并进行了表征。PP(i)-PFK的独特之处在于，其米氏常数（K(m)）和最大反应速度（V(max)）值表明，多聚磷酸是比焦磷酸更优的底物；实际上该酶是一种多聚磷酸依赖性PFK。ATP-PFK不受常见变构效应剂（如磷酸烯醇丙酮酸）的显著影响，但受到PP(i)和多聚磷酸的强烈抑制。结果表明，该生物体中糖酵解途径的调控可能受焦磷酸和/或多聚磷酸的调节。
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