[56株苏云金芽孢杆菌分离株中毒素蛋白基因的鉴定与定位]
[Identification and location of the toxin protein genes in 56 Bacillus thuringiensis isolates].
作者信息
Zhong X, Yuan M, Zhang P, Deng R, Long Q, Pang Y, Wang X
机构信息
Institute of Entomology and State Key Laboratory for Biocontrol, Zhongshan University, Guangzhou 510275, China.
出版信息
Wei Sheng Wu Xue Bao. 2001 Jun;41(3):293-7.
Polymerase chain reaction was performed to screen Bacillus thuringiensis isolates for cryI, cryIII, and cryV genes with cryI-, cryIII-, and cryV-specific primers respectively. The results indicated that, in the 56 Bt isolates examined, 7 isolates contain cryI gene, 2 isolates contain cryIII gene, and 21 isolates contain both cryI and cryV genes. The cryV gene was located by Southern hybridization in the large plasmid of about 150 MD molecular mass.
分别使用cryI、cryIII和cryV特异性引物进行聚合酶链反应，以筛选苏云金芽孢杆菌分离株中的cryI、cryIII和cryV基因。结果表明，在所检测的56株Bt分离株中，7株含有cryI基因，2株含有cryIII基因，21株同时含有cryI和cryV基因。通过Southern杂交将cryV基因定位在大约150 MD分子质量的大质粒上。
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