Nishiyama T, Amano S, Tsunenaga M, Kadoya K, Takeda A, Adachi E, Burgeson R E
Shiseido Life Science Research Center, Yokohama, Japan. toshio.nishiyama@to,shiseido.co.jp
J Dermatol Sci. 2000 Dec;24 Suppl 1:S51-9. doi: 10.1016/s0923-1811(00)00142-0.
The skin consists of two main layers, epidermis and dermis, separated by the basement membrane. Epidermal-dermal communication through the basement membrane is important for skin homeostasis. The basement membrane contains specialized structures, called the anchoring complex, which ensure the stability of connection and communication between these two tissue compartments. The proteins within the anchoring complex provide links to both the intracellular cytoskeletal keratins in keratinocytes and connective tissue proteins of the dermis. One of the key components of the complex is laminin 5, which is essential to epidermal cell attachment. The biological function of laminin 5 has been investigated by using a skin equivalent model in vitro and during keratinocyte sheet grafting in vivo. As a major link between the epidermal basal cells and the papillary dermis, laminin 5 initiates hemidesmosome formation and provides stable attachment of the epidermis to the dermis. Laminin 5 also accelerates the assembly of basement membranes and may enhance the recovery of damaged skin. An intact basement membrane at the epidermal-dermal junction is essential to stability of the skin.
皮肤由两个主要层组成，即表皮和真皮，它们由基底膜分隔。通过基底膜进行的表皮 - 真皮通讯对于皮肤稳态很重要。基底膜包含称为锚定复合体的特殊结构，这些结构确保了这两个组织隔室之间连接和通讯的稳定性。锚定复合体内的蛋白质与角质形成细胞内的细胞内细胞骨架角蛋白和真皮的结缔组织蛋白都有联系。该复合体的关键成分之一是层粘连蛋白5，它对表皮细胞附着至关重要。层粘连蛋白5的生物学功能已通过体外使用皮肤等效模型和体内角质形成细胞片移植进行了研究。作为表皮基底细胞和乳头真皮之间的主要连接，层粘连蛋白5启动半桥粒形成并为表皮与真皮提供稳定附着。层粘连蛋白5还加速基底膜的组装，并可能增强受损皮肤的恢复。表皮 - 真皮交界处完整的基底膜对于皮肤的稳定性至关重要。
