Albouy Marion, Santos Morgan Dos, Laho Kilian, Couchourel Denis, Tranchepain Frédéric
Laboratoires VIVACY, France.
LabSkin Creations, Lyon, France.
J Cosmet Dermatol. 2025 Sep;24(9):e70362. doi: 10.1111/jocd.70362.
Superficial injection of hyaluronic acid (HA)-based gels is a widely used method to restore skin quality and achieve a more youthful appearance. While the clinical benefits of such procedures are well established, their biological mechanisms of action remain poorly understood.
This study aimed to evaluate the effectiveness of two cross-linked HA gels (IPN-12.5 and HA-X12) on skin rejuvenation, focusing on skin hydration and extracellular matrix quality. The evaluation was conducted using an innovative skin model that closely mimics native human skin.
A bioengineered scaffold composed of collagen, glycosaminoglycans, and chitosan was developed and seeded with aged human fibroblasts. This seeded scaffold has the ability to allow cellular migration, proliferation, and neo-synthesize its own extracellular matrix. Normal human keratinocytes were subsequently seeded on top of the newly formed dermal equivalent, thereby simulating the complex interactions that occur between the epidermis and dermis. Multipoint intradermal injections of the test products were administrated using a multipoint technique during the reconstruction protocol.
Both products significantly decreased transepidermal water loss (TEWL), though histomorphological differences were observed. The IPN-12.5-hydrogel-injected models showed enhanced barrier integrity, water retention, epidermal renewal, and skin elasticity markers compared to the controls and HA-X12.
IPN-12.5 hydrogel significantly improved hydration and elasticity-related skin markers when compared to a similar product (HA-X12). Despite similar clinical indications and concentrations, the products exhibited different biological effects, potentially due to differences in cross-linking technologies or HA molecular weights. These factors are likely to influence clinical outcomes achieved with the two formulations.
基于透明质酸（HA）的凝胶进行浅表注射是一种广泛应用的恢复皮肤质量和实现更年轻外观的方法。虽然此类手术的临床益处已得到充分证实，但其生物学作用机制仍知之甚少。
本研究旨在评估两种交联HA凝胶（IPN - 12.5和HA - X12）对皮肤年轻化的有效性，重点关注皮肤水合作用和细胞外基质质量。使用一种紧密模拟天然人类皮肤的创新皮肤模型进行评估。
开发了一种由胶原蛋白、糖胺聚糖和壳聚糖组成的生物工程支架，并接种老化的人类成纤维细胞。这种接种后的支架能够允许细胞迁移、增殖并重新合成其自身的细胞外基质。随后将正常人角质形成细胞接种在新形成的真皮替代物顶部，从而模拟表皮和真皮之间发生的复杂相互作用。在重建过程中，使用多点技术对测试产品进行多点皮内注射。
两种产品均显著降低了经表皮水分流失（TEWL），不过观察到了组织形态学差异。与对照组和HA - X12相比，注射IPN - 12.5水凝胶的模型显示出屏障完整性、保水性、表皮更新和皮肤弹性标志物增强。
与类似产品（HA - X12）相比，IPN - 12.5水凝胶显著改善了与水合作用和弹性相关的皮肤标志物。尽管临床适应症和浓度相似，但由于交联技术或HA分子量的差异，这两种产品表现出不同的生物学效应。这些因素可能会影响两种制剂所取得的临床结果。
