器官型海马体培养物。肺炎链球菌脑膜炎中脑组织损伤的一种模型。

Organotypic hippocampal cultures. A model of brain tissue damage in Streptococcus pneumoniae meningitis.

作者信息

Schmidt H, Tlustochowska A, Stuertz K, Djukic M, Gerber J, Schütz E, Kuhnt U, Nau R

机构信息

Department of Neurology, University of Goettingen, Robert-Koch Strasse 40, D-37075 Goettingen, Germany.

出版信息

J Neuroimmunol. 2001 Feb 1;113(1):30-9. doi: 10.1016/s0165-5728(00)00402-1.

DOI:10.1016/s0165-5728(00)00402-1

PMID:11137574

Abstract

Hippocampal slices of newborn rats were exposed to either heat-inactivated Streptococcus pneumoniae R6 (hiR6) equivalent to 10(6) and 10(8) CFU/ml, lipoteichoic acid (LTA) (0.3 microg/ml and 30 microg/ml), peptidoglycans (PG) (0.3, 30, 50 and 100 microg/ml), pneumococcal DNA (pDNA) (0.3 and 30 microg/ml) or medium only (control). Cell injury was examined by Nissl staining, Annexin V and NeuN immunohistochemistry, and quantified by propidium iodide (PI) uptake and by determining neuron-specific enolase (NSE) concentration in the culture medium. Necrotic and apoptotic cell damage occurred in all treatment groups. Overall damage (Nissl and PI staining) was most prominent after hiR6 (10(8) CFU/ml), followed by LTA (30 microg/ml), pDNA (30 microg/ml), and not detectable after PG (30 microg/ml) exposure. PG (100 microg/ml) induced severe damage. Apoptotic cells were most frequent after exposure to LTA and hiR6. Damage in the neuronal cell layers (NeuN, NSE) was most severe after treatment with hiR6 (10(8) CFU/ml), followed by PG (100 microg/ml), pDNA (30 microg/ml), and LTA (30 microg/ml).

摘要

将新生大鼠的海马切片暴露于相当于10⁶和10⁸CFU/ml的热灭活肺炎链球菌R6（hiR6）、脂磷壁酸（LTA）（0.3μg/ml和30μg/ml）、肽聚糖（PG）（0.3、30、50和100μg/ml）、肺炎球菌DNA（pDNA）（0.3和30μg/ml）或仅培养基（对照）中。通过尼氏染色、膜联蛋白V和NeuN免疫组织化学检查细胞损伤，并通过碘化丙啶（PI）摄取和测定培养基中神经元特异性烯醇化酶（NSE）浓度进行量化。所有治疗组均发生坏死和凋亡性细胞损伤。总体损伤（尼氏染色和PI染色）在hiR6（10⁸CFU/ml）后最为明显，其次是LTA（30μg/ml）、pDNA（30μg/ml），PG（30μg/ml）暴露后未检测到损伤。PG（100μg/ml）诱导严重损伤。暴露于LTA和hiR6后凋亡细胞最为常见。用hiR6（10⁸CFU/ml）处理后，神经元细胞层（NeuN、NSE）的损伤最为严重，其次是PG（100μg/ml）、pDNA（30μg/ml）和LTA（30μg/ml）。