通过对cag致病岛进行简单基因分析来确定幽门螺杆菌的毒力
Determination of Helicobacter pylori virulence by simple gene analysis of the cag pathogenicity island.
作者信息
Ikenoue T, Maeda S, Ogura K, Akanuma M, Mitsuno Y, Imai Y, Yoshida H, Shiratori Y, Omata M
机构信息
Department of Gastroenterology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
出版信息
Clin Diagn Lab Immunol. 2001 Jan;8(1):181-6. doi: 10.1128/CDLI.8.1.181-186.2001.
Nucleic acid amplification was performed for five loci in the cag pathogenicity island (PAI) of Helicobacter pylori (comprising cagA, the cagA promoter region, cagE, cagT, and the left end of cagII [LEC]), and gastric inflammation in patients was evaluated. Of 204 H. pylori isolates from Japanese patients (53 with peptic ulcer, 55 with gastric cancer, and 96 with chronic gastritis), 197 (96.6%) were positive for all five loci. Two isolates (1%) were negative for all five loci, and five isolates (2.4%) were positive for only cagA and LEC. These latter seven isolates were all from patients with mild chronic gastritis. Neutrophil infiltration in gastric mucosa was significantly milder in patients infected with partially or totally deleted-PAI strains than in those with intact-PAI strains. The cagE gene was a more accurate marker of an intact cag PAI than the cagA gene, and cagE seemed to be more useful in discriminating between H. pylori strains causing different rates of disease progression.
对幽门螺杆菌空泡毒素相关基因（cag）致病岛中的五个位点（包括cagA、cagA启动子区域、cagE、cagT以及cagII左端[LEC]）进行核酸扩增，并评估患者的胃部炎症情况。在来自日本患者的204株幽门螺杆菌分离株中（53例患有消化性溃疡，55例患有胃癌，96例患有慢性胃炎），197株（96.6%）这五个位点均呈阳性。2株分离株（1%）这五个位点均为阴性，5株分离株（2.4%）仅cagA和LEC呈阳性。后七株分离株均来自轻度慢性胃炎患者。与感染完整cag致病岛菌株的患者相比，感染部分或完全缺失cag致病岛菌株的患者胃黏膜中的中性粒细胞浸润明显较轻。与cagA基因相比，cagE基因是完整cag致病岛更准确的标志物，并且cagE似乎在区分导致不同疾病进展率的幽门螺杆菌菌株方面更有用。
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