Maeda S, Kanai F, Ogura K, Yoshida H, Ikenoue T, Takahashi M, Kawabe T, Shiratori Y, Omata M
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Dig Dis Sci. 1997 Sep;42(9):1841-7. doi: 10.1023/a:1018846723379.
CagA-positive H. pylori is reported to be associated with gastroduodenal disease in Western countries. To evaluate the relationship between CagA and disease, cloning of the entire cagA gene (3771 bp), insertion of a partial fragment (1272 bp) into an expression vector, purification of the recombinant protein, production of an antibody against the recombinant CagA protein through rabbits, and use of the recombinant CagA protein as an antigen, detection of the anti-CagA antibody by western blotting were all performed. Sera of 132 H. pylori-infected patients undergoing endoscopy were studied. Anti-CagA antibodies were detected in 90%, 87%, 90%, 94%, and 93% of patients with gastric ulcer (N = 34), duodenal ulcer (N = 27), chronic gastritis (N = 31), gastric cancer (N = 17), and normal mucosa (N = 15), respectively. High seropositivity of anti-CagA antibody even in individuals with normal mucosa indicated that CagA may not be a unique marker for disease by H. pylori infection in Japan.
据报道，在西方国家，细胞毒素相关基因A（CagA）阳性的幽门螺杆菌与胃十二指肠疾病有关。为了评估CagA与疾病之间的关系，进行了以下操作：克隆整个cagA基因（3771 bp），将部分片段（1272 bp）插入表达载体，纯化重组蛋白，通过兔子产生针对重组CagA蛋白的抗体，并使用重组CagA蛋白作为抗原，通过蛋白质印迹法检测抗CagA抗体。研究了132例接受内镜检查的幽门螺杆菌感染患者的血清。胃溃疡（N = 34）、十二指肠溃疡（N = 27）、慢性胃炎（N = 31）、胃癌（N = 17）和正常黏膜（N = 15）患者中抗CagA抗体的检测率分别为90%、87%、90%、94%和93%。即使在正常黏膜个体中抗CagA抗体也具有较高的血清阳性率，这表明在日本，CagA可能不是幽门螺杆菌感染所致疾病独特的标志物。
