Drize N I, Drutskaya M S, Gerasimova L P, Manakova T E, Chertkov I L, Turetskaya R L, Kuprash D V, Nedospasov S A
Hematology Research Center, Russian Academy of Medical Sciences, Moscow.
Bull Exp Biol Med. 2000 Jul;130(7):676-8. doi: 10.1007/BF02682103.
Hemopoietic and stromal precursor cells were studied in mice deficient for tumor necrosis factor or lymphotoxin-alpha. In normal hemopoiesis the main characteristics of hemopoiesis in knockout mice did not differ from those in wild-type mice. Implantation of bone marrow cells from mice deficient for tumor necrosis factor onto irradiated sublayer of a long-living bone marrow culture led to a notable increase in the number of mature cells and granulocytic-macrophage precursor cells. This can be due to the fact that tumor necrosis factor inhibits proliferation of hemopoietic precursor cells, while in the absence of this factor precursor cells actively proliferate. On the other hand, cell composition and number of colony-forming units of granulocytes-macrophages are significantly decreased in cultures onto which bone marrow cells from lymphotoxin-alpha-deficient mice were implanted. This can be explained by impaired expression of adhesion molecules in these animals. In addition, the number of stromal precursor cells was changed in mice deficient by genes of the tumor necrosis factor cluster.
在缺乏肿瘤坏死因子或淋巴毒素α的小鼠中研究了造血和基质前体细胞。在正常造血过程中，基因敲除小鼠造血的主要特征与野生型小鼠没有差异。将缺乏肿瘤坏死因子的小鼠的骨髓细胞植入长寿骨髓培养物的照射亚层上，导致成熟细胞和粒细胞-巨噬细胞前体细胞数量显著增加。这可能是由于肿瘤坏死因子抑制造血前体细胞的增殖，而在没有该因子的情况下，前体细胞会积极增殖。另一方面，植入了缺乏淋巴毒素α的小鼠的骨髓细胞的培养物中，粒细胞-巨噬细胞集落形成单位的细胞组成和数量显著减少。这可以通过这些动物中黏附分子表达受损来解释。此外，缺乏肿瘤坏死因子簇基因的小鼠中基质前体细胞的数量发生了变化。
