Nifontova I N, Ershler M A, Drize N I
Research Center for Hematology, Russian Academy of Medical Sciences, Moscow.
Bull Exp Biol Med. 2002 Apr;133(4):386-8. doi: 10.1023/a:1016210407784.
The total duration of hemopoiesis and the total cell production in long-term bone marrow cultures from mice deficient by tumor necrosis factor are increased, while proliferation of granulocyte-macrophage precursor cells, the main cell populations in long-term bone marrow cultures, did not differ from that in wild-type mice. In bone marrow cultures from knockout mice the intensity of apoptosis remained low during 40-week culturing and was similar to that in early wild-type cultures (10 weeks). Then, the intensity of apoptosis in bone marrow cultures from knockout mice did not differ or even surpassed that in wild-type cultures. However, we observed no inhibition of hemopoiesis in bone marrow cultures from knockout mice. The absence of autocrine expression of tumor necrosis factor did not affect proliferation of precursor cells, but modulate the intensity of apoptosis. It remains unclear whether changes in apoptosis are related to intensive cell production.
肿瘤坏死因子缺陷小鼠长期骨髓培养中造血的总持续时间和总细胞产量增加，而长期骨髓培养中的主要细胞群体粒细胞 - 巨噬细胞前体细胞的增殖与野生型小鼠无异。在基因敲除小鼠的骨髓培养中，凋亡强度在40周培养期间一直较低，与早期野生型培养（10周）相似。然后，基因敲除小鼠骨髓培养中的凋亡强度与野生型培养无差异，甚至超过野生型培养。然而，我们在基因敲除小鼠的骨髓培养中未观察到造血抑制。肿瘤坏死因子自分泌表达的缺失不影响前体细胞的增殖，但调节凋亡强度。目前尚不清楚凋亡的变化是否与细胞大量产生有关。
