Nifontova I N, Ershler M A, Drize N I
Hematological Research Center, Russian Academy of Medical Sciences, Moscow.
Bull Exp Biol Med. 2003 Mar;135(3):285-8. doi: 10.1023/a:1024149401356.
Hemopoiesis in a long-term bone marrow culture derived from mice deficient for tumor necrosis factor was maintained for more than 130 weeks, which was 4 times longer than in cultures from wild type mice. The dynamics of hemopoietic precursor cells of different maturity was studied in the suspension fraction of the culture. The incidence of granulocyt-macrophage precursor cells and cell forming cobblestone areas was studied by the method of limiting dilutions in culture. In contrast to cultures derived from wild type mice, in long-term bone marrow culture derived from tumor necrosis factor deficient mice, first, the incidence of early precursor cells gradually increased and then the incidence of all precursor cells sharply increased.
来自肿瘤坏死因子缺陷小鼠的长期骨髓培养中的造血功能维持了超过130周，这比野生型小鼠培养物中的造血功能维持时间长4倍。在培养物的悬浮部分研究了不同成熟度的造血前体细胞的动态变化。通过培养中的极限稀释法研究了粒细胞-巨噬细胞前体细胞和形成鹅卵石区域的细胞的发生率。与来自野生型小鼠的培养物相比，在来自肿瘤坏死因子缺陷小鼠的长期骨髓培养物中，首先，早期前体细胞的发生率逐渐增加，然后所有前体细胞的发生率急剧增加。
