O'Connor P B, Costello C E
Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118, USA.
Anal Chem. 2000 Dec 15;72(24):5881-5. doi: 10.1021/ac000770t.
Using matrix-assisted laser desorption/ionization (MAL DI) on a trapped ion mass spectrometer such as a Fourier transform mass spectrometer (FTMS) allows accumulation of ions in the cell from multiple laser shots prior to detection. If ions from separate MALDI samples are accumulated simultaneously in the cell, ions from one sample can be used to calibrate ions from the other sample. Since the ions are detected simultaneously in the cell, this is, in effect, internal calibration, but there are no selective desorption effects in the MALDI source. This method of internal calibration with adjacent samples is demonstrated here on cesium iodide clusters, peptides, oligosaccharides, poly(propylene glycol), and fullerenes and provides typical FTMS internal calibration mass accuracy of < 1 ppm.
在诸如傅里叶变换质谱仪（FTMS）之类的捕获离子质谱仪上使用基质辅助激光解吸/电离（MALDI），可在检测前通过多次激光照射在细胞中积累离子。如果来自单独的MALDI样品的离子同时在细胞中积累，则一个样品的离子可用于校准另一个样品的离子。由于离子在细胞中同时被检测到，这实际上是内部校准，但在MALDI源中不存在选择性解吸效应。本文在碘化铯簇、肽、寡糖、聚丙二醇和富勒烯上展示了这种相邻样品内部校准的方法，并提供了典型的FTMS内部校准质量准确度<1 ppm。
