通过亲和细胞捕获质谱法分析成年黑腹果蝇中枢神经系统中的神经肽表达和定位。
Analysis of neuropeptide expression and localization in adult drosophila melanogaster central nervous system by affinity cell-capture mass spectrometry.
作者信息
Yew Joanne Y, Wang Yun, Barteneva Natasha, Dikler Sergei, Kutz-Naber Kimberly K, Li Lingjun, Kravitz Edward A
机构信息
Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA.
出版信息
J Proteome Res. 2009 Mar;8(3):1271-84. doi: 10.1021/pr800601x.
Abstract
A combined approach using mass spectrometry, a novel neuron affinity capture technique, and Drosophila melanogaster genetic manipulation has been developed to characterize the expression and localization of neuropeptides in the adult D. melanogaster brain. In extract from the whole adult brain, 42 neuropeptides from 18 peptide families were sequenced. Neuropeptide profiling also was performed on targeted populations of cells which were enriched with immunoaffinity purification using a genetically expressed marker.
摘要
已开发出一种结合使用质谱分析、一种新型神经元亲和捕获技术和黑腹果蝇基因操作的方法，以表征成年黑腹果蝇大脑中神经肽的表达和定位。在整个成年大脑的提取物中，对来自18个肽家族的42种神经肽进行了测序。还对使用基因表达标记通过免疫亲和纯化富集的靶向细胞群体进行了神经肽谱分析。
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