Murphy J M, Browne R W, Hill L, Bolelli G F, Abagnato C, Berrino F, Freudenheim J, Trevisan M, Muti P
Department of Social and Preventive Medicine, State University of New York at Buffalo, Buffalo, NY 14214, USA.
Nutr Cancer. 2000;37(2):155-60. doi: 10.1207/S15327914NC372_6.
The effects of transportation and delay in processing of blood samples on the concentration of biomarkers are significant in epidemiological studies for which specimens are collected from participants at locations other than a designated center or laboratory. These sources of variability in measurement were studied by collecting two sets of blood samples from 51 men between 26 and 50 years of age. The first set was sent immediately to the laboratory for processing. The second set was transported by car for one hour and then returned to the laboratory for processing. Both sets were stored together at -80 degrees C until the end of the study. Several blood constituents were evaluated. Vitamins, liver enzymes, and electrolytes showed no changes in concentration after transport by car for one hour. There were decreases in the concentrations of red and white blood cells, high-density-lipoprotein cholesterol, glucose, and creatinine after transportation. The transported total cholesterol, total testosterone, free testosterone, alkaline phosphatase, total bilirubin, and thiobarbituric acid-reactive substances increased in concentration. Although transportation and delay in processing of blood samples do not appear to greatly impact relative risk estimates, epidemiologists should be aware of these potential sources of variability in measurement and consider the consequences in their particular study.
在流行病学研究中，对于那些在指定中心或实验室以外的地点从参与者身上采集标本的研究而言，血液样本的运输及处理延迟对生物标志物浓度的影响颇为显著。通过从51名年龄在26至50岁之间的男性身上采集两组血液样本，对这些测量中的变异性来源进行了研究。第一组样本立即送往实验室进行处理。第二组样本通过汽车运输一小时，然后返回实验室进行处理。两组样本都在-80摄氏度下共同储存，直至研究结束。对几种血液成分进行了评估。维生素、肝酶和电解质在通过汽车运输一小时后浓度未出现变化。运输后红细胞、白细胞、高密度脂蛋白胆固醇、葡萄糖和肌酐的浓度有所下降。运输后的总胆固醇、总睾酮、游离睾酮、碱性磷酸酶、总胆红素和硫代巴比妥酸反应性物质的浓度有所增加。尽管血液样本的运输及处理延迟似乎对相对风险估计没有太大影响，但流行病学家应意识到这些潜在的测量变异性来源，并在其特定研究中考虑其后果。
