Mitchell G V, Cook K K, Jenkins M Y, Grundel E
Office of Nutritional Products, Labeling and Dietary Supplements, Center for Food Safety and Applied Nutrition, Food and Drug Administration, 8301 Muirkirk Road, Laurel, MD 20708, USA.
Int J Vitam Nutr Res. 2001 Jan;71(1):30-5. doi: 10.1024/0300-9831.71.1.30.
The modulation of tissue concentrations of vitamin K by a lutein supplement preserved with natural vitamin E was studied in Fischer 344 rats. Vitamin K is necessary for blood coagulation and may be essential for tissue and bone health. Weanling male rats were fed the AIN-93G diet (control) or modified AIN-93G diets containing 0.3, 0.6, 1.2, 2.4 and 4.8 g supplement/100 g diet for 8 weeks. The supplement contained 5% lutein, 0.22% zeaxanthin and 2.2% natural vitamin E as a preservative. Concentrations of trans-phylloquinone in the plasma (nmol/mmol triglycerides) and heart were significantly reduced (P < or = 0.05) in rats fed the supplement. The reductions in trans-phylloquinone in the heart ranged from approximately 20 to 60% of the control. Concentrations of phylloquinone in the liver were significantly lower in the rats fed the supplement at levels > or = 1.2 g/100 g diet than in the control rats. Ratios of cis/trans phylloquinone in liver and heart increased and concentrations of menaquinone-4 in heart decreased as the dietary level of the lutein supplement increased. The results suggest that the lutein supplement affected the absorption, tissue uptake and/or turnover rate of vitamin K. The presence of other components in the supplement confounded the interpretation of the biological effects of lutein alone on vitamin K metabolism.
在Fischer 344大鼠中研究了用天然维生素E保存的叶黄素补充剂对维生素K组织浓度的调节作用。维生素K对血液凝固是必需的，可能对组织和骨骼健康至关重要。将断奶雄性大鼠喂食AIN-93G饮食（对照）或含有0.3、0.6、1.2、2.4和4.8 g补充剂/100 g饮食的改良AIN-93G饮食，持续8周。该补充剂含有5%的叶黄素、0.22%的玉米黄质和2.2%的天然维生素E作为防腐剂。喂食补充剂的大鼠血浆（nmol/mmol甘油三酯）和心脏中的反式叶绿醌浓度显著降低（P≤0.05）。心脏中反式叶绿醌的降低幅度为对照的约20%至60%。喂食补充剂且水平≥1.2 g/100 g饮食的大鼠肝脏中叶绿醌浓度显著低于对照大鼠。随着叶黄素补充剂饮食水平的增加，肝脏和心脏中顺式/反式叶绿醌的比例增加，心脏中甲基萘醌-4的浓度降低。结果表明，叶黄素补充剂影响了维生素K的吸收、组织摄取和/或周转率。补充剂中其他成分的存在混淆了单独叶黄素对维生素K代谢的生物学效应的解释。
