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酿酒酵母线粒体甘油-3-磷酸脱氢酶基因GUT2的碳源依赖性转录调控

Carbon source-dependent transcriptional regulation of the mitochondrial glycerol-3-phosphate dehydrogenase gene, GUT2, from Saccharomyces cerevisiae.

作者信息

Grauslund M, Rønnow B

机构信息

Danisco Cultor Innovation, Copenhagen, Denmark.

出版信息

Can J Microbiol. 2000 Dec;46(12):1096-100. doi: 10.1139/w00-105.

DOI:10.1139/w00-105
PMID:11142398
Abstract

Cytosolic glycerol kinase (Gut1p) and mitochondrial glycerol-3-phosphate dehydrogenase (Gut2p) constitute the glycerol utilization pathway in Saccharomyces cerevisiae. Transcriptional analysis of the GUT2 gene showed that it was repressed by glucose and derepressed on the non-fermentable carbon sources, glycerol, lactate and ethanol. Derepression of GUT2 requires the protein kinase Snflp as well as the heteromeric protein complex, Hap2/3/4/5, and its putative DNA-binding site (UASHAP) located in the promoter region. Furthermore, glucose repression of GUT2 requires the negative regulator, Opi1p.

摘要

胞质甘油激酶（Gut1p）和线粒体甘油 -3-磷酸脱氢酶（Gut2p）构成了酿酒酵母中的甘油利用途径。对GUT2基因的转录分析表明，它受到葡萄糖的抑制，而在非发酵性碳源（甘油、乳酸和乙醇）上则去抑制。GUT2的去抑制需要蛋白激酶Snflp以及异源蛋白复合物Hap2/3/4/5，及其位于启动子区域的假定DNA结合位点（UASHAP）。此外，GUT2的葡萄糖抑制需要负调节因子Opi1p。

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