Sleep D, Ogden J E, Roberts N A, Goodey A R
Delta Biotechnology Limited, Nottingham, U.K.
Gene. 1991 May 15;101(1):89-96. doi: 10.1016/0378-1119(91)90228-4.
The Saccharomyces cerevisiae glycerol-3-phosphate dehydrogenase (GUT2) promoter and part of the protein-coding region have been isolated on a 6.3-kb genomic DNA fragment. Nucleotide sequence analysis shows that the promoter has many structural features in common with yeast glycolytic enzyme promoters. Chromosomal mapping indicates that this genomic fragment is located on chromosome XII. The GUT2 promoter has been used to construct a recombinant human albumin (reHA) secretion vector; yeast transformed with this vector secrete reHA into the culture supernatant.
酿酒酵母甘油 - 3 - 磷酸脱氢酶（GUT2）启动子及部分蛋白质编码区已在一个6.3 kb的基因组DNA片段上分离出来。核苷酸序列分析表明，该启动子具有许多与酵母糖酵解酶启动子共有的结构特征。染色体定位显示，这个基因组片段位于第十二号染色体上。GUT2启动子已被用于构建重组人白蛋白（reHA）分泌载体；用该载体转化的酵母将reHA分泌到培养上清液中。
