Pearson D B, Goodman R, Sachs H
Science. 1975 Mar 21;187(4181):1081-2. doi: 10.1126/science.1114335.
The hypothalamo-neurohypophyseal complex (HNC) of the fetal guinea pig shows a dramatic increase in its content of vasopressin and neurophysin between the 40th and 55th days of gestation. The values for radioimmunoassayable hormone and binding protein are at day 40, 2 milliunits and less than 0.1 microgram; and at day 55, about 100 milliunits and 10 micrograms, respectively. Isotope incorporation experiments with organ cultures of the fetal HNC taken prior to the 35th day of gestation added additional confirmation of the inability of the hypothalamic neurosecretory cells to synthesize vasopressin or neurophysin at this time. However, by the 45th day, similar organ cultures show a vigorous incorporation of labeled amino acids into both hormone and binding protein. Furthermore, the HNC of the 45-day-old fetus apparently contains a factor that stimulates specifically the biosynthesis of vasopressin and neurophysin in HNC cultures from the adult guinea pig. This factor is not detectable in either cortex or liver of the 45-day-old fetus or in the fetal HNC taken prior to or after the period of exponential rise (40th to 55th days) of hormone and binding protein.
豚鼠胎儿的下丘脑 - 神经垂体复合体（HNC）在妊娠第40天到第55天期间，其抗利尿激素和神经垂体素的含量急剧增加。放射免疫分析可检测的激素和结合蛋白的值在第40天分别为2毫单位和小于0.1微克；在第55天分别约为100毫单位和10微克。对妊娠第35天之前获取的胎儿HNC进行器官培养的同位素掺入实验，进一步证实了此时下丘脑神经分泌细胞无法合成抗利尿激素或神经垂体素。然而，到第45天，类似的器官培养显示标记氨基酸大量掺入激素和结合蛋白中。此外，45日龄胎儿的HNC显然含有一种因子，该因子能特异性刺激成年豚鼠HNC培养物中抗利尿激素和神经垂体素的生物合成。在45日龄胎儿的皮质或肝脏中，以及在激素和结合蛋白呈指数上升期（第40天至第55天）之前或之后获取的胎儿HNC中，均未检测到该因子。
