Marson A M, Privat A
Cell Tissue Res. 1979;203(3):393-401. doi: 10.1007/BF00233269.
The development of neurophysin-oxytocin and neurophysin-vasopressin containing neurons of the guinea pig was studied in vitro. Supraoptic (SO) and paraventricular (PV) nuclei were explanted from guinea pig foetuses at the 40th day of gestation and cultured in Maximov slides for nearly fifty days. The cultures were observed daily under a phase-contrast microscope. Explants were fixed every five days for observation with the electron microscope. At the time of explantation, magnocellular neurons were still immature. They acquired the morphologic characteristics of mature neurons, with axosomatic synapses, after about 10 days in vitro. After 15-20 days in vitro, they contained in addition neurosecretory granules (NSG), first in the Golgi region, then also dispersed in the cytoplasm. In the oldest culture (45-50 days), signs of granulolysis were regularly found. It appears that magnocellular neurosecretory neurons are able to differentiate in vitro from a primitive state in the absence of specific stimulation.
对豚鼠中含有神经垂体素 - 催产素和神经垂体素 - 抗利尿激素的神经元的发育进行了体外研究。在妊娠第40天从豚鼠胎儿中取出视上核（SO）和室旁核（PV），并在马克西莫夫载玻片上培养近50天。每天在相差显微镜下观察培养物。每隔5天固定外植体用于电子显微镜观察。在植入时，大细胞神经元仍未成熟。在体外培养约10天后，它们获得了成熟神经元的形态学特征，出现了轴体突触。在体外培养15 - 20天后，它们还含有神经分泌颗粒（NSG），首先出现在高尔基体区域，然后也分散在细胞质中。在最老的培养物（45 - 50天）中，经常发现颗粒溶解的迹象。看来大细胞神经分泌神经元在没有特定刺激的情况下能够在体外从原始状态分化。
