Saulis G
Department of Biology, Vytautas Magnus University, 28 Daukanto, Kaunas 3000, Lithuania.
Biomed Sci Instrum. 1999;35:291-6.
In this paper the size and the apparent number of the pores induced in human red blood cells under the influence of a single electric field pulse have been estimated. The size of the pores was evaluated by two different approaches: (i) by measuring the kinetics of ascorbic acid entrance into the cells and (ii) by studying the protective action of mannitol and sucrose against colloid-osmotic lysis. The data presented here indicated that (i) the pores are small (0.2 < r < 0.5 nm); (ii) pores with a size such that the molecules of ascorbic acid and mannitol could penetrate through them appear when the field strength exceeds 2.5 kV/cm (tau i = 20 microseconds); (iii) with increasing pulse intensity there is an increase in both the radius of the pores and the number of cells permeable to mannitol or sucrose. It has been concluded that the presence of only one small (r approximately 0.3 nm) pore in the human erythrocyte is sufficient for a cell to be regarded as porated.
本文估算了在单个电场脉冲作用下人体红细胞中诱导产生的孔的大小和表观数量。通过两种不同方法评估孔的大小：（i）测量抗坏血酸进入细胞的动力学；（ii）研究甘露醇和蔗糖对胶体渗透裂解的保护作用。此处给出的数据表明：（i）孔很小（0.2＜r＜0.5纳米）；（ii）当场强超过2.5千伏/厘米（脉冲宽度τi = 20微秒）时，会出现大小足以使抗坏血酸和甘露醇分子穿过的孔；（iii）随着脉冲强度增加，孔的半径以及对甘露醇或蔗糖可渗透的细胞数量均增加。得出的结论是，人体红细胞中仅存在一个小（r约为0.3纳米）孔就足以使细胞被视为有孔。
