Saulis G
Department of Biology, Vytautas Magnus University, 28 Daukanto, Kaunas 3000, Lithuania.
Biomed Sci Instrum. 1999;35:409-14.
The process of pore disappearance after cell electroporation is analysed theoretically. On the basis of the kinetic model, in which the formation and annihilation of a metastable hydrophilic pore are considered as random one-step processes, a distribution function of cell resealing times, Fr(t), is derived. The shape of this function depends on the initial number of pores in a cell, ni. The developed theory is compared with experimental data. Although the shape of experimental relationships depends on the electroporation conditions, they can be described by theoretical curves quite well. Increasing the amplitude of an electric pulse increases the number of pores and/or the rate of their resealing. It was concluded that the disappearance of pores in the cell membrane after electroporation is a random process.
从理论上分析了细胞电穿孔后孔隙消失的过程。基于动力学模型，其中亚稳态亲水孔隙的形成和湮灭被视为随机的一步过程，推导出了细胞重新封闭时间的分布函数(F_r(t))。该函数的形状取决于细胞中初始孔隙的数量(n_i)。将所提出的理论与实验数据进行了比较。尽管实验关系的形状取决于电穿孔条件，但它们可以很好地用理论曲线来描述。增加电脉冲的幅度会增加孔隙的数量和/或它们重新封闭的速率。得出的结论是，电穿孔后细胞膜中孔隙的消失是一个随机过程。
