Simultaneous determination of vitamins A, D3 and E in multiple pharmaceutical preparations by HPLC method.

This study makes possible the use of reversed phase HPLC with detection at 265 nm for the simultaneous quantitative determination of vitamin A, D3 and E in multiple pharmaceutical preparations. We have also proposed a very simple method of isolation of these vitamins from multivitamin preparations. On the basis of statistical analysis of the data, we can note that conditions for HPLC method used in our work gave us precise and accurate results.