Chávez-Servín Jorge L, Castellote Ana I, López-Sabater M Carmen
Dpt. de Nutrició i Bromatologia, Centre de Referència en Tecnologia dels Aliments (CeRTA), Facultat de Farmàcia, Universitat de Barcelona, Avda. Joan XXIII s/n, E-08028 Barcelona, Spain.
J Chromatogr A. 2006 Jul 28;1122(1-2):138-43. doi: 10.1016/j.chroma.2006.04.059. Epub 2006 May 11.
A rapid, simple and reproducible normal-phase (NP) high-performance liquid chromatography (HPLC)-diode array detection (DAD) method for simultaneous qualitative and quantitative determination of Vitamin A (retinol acetate and retinol palmitate) and Vitamin E (alpha-tocopherol acetate, alpha-, gamma- and delta-tocopherols) in milk-based infant formulae was developed and validated. The preparation sample was based on protein precipitation and vitamin extraction with ethanol, followed by re-extraction with hexane, while the chromatographic method was based on the use of a short narrow-bore column (50 mm x 2.1 mm; 3 microm particle size), which afforded less solvent consumption and higher mass sensitivity. The method showed acceptable values for precision, recovery and sensitivity, and proved very simple for routine analysis work.
建立并验证了一种快速、简单且可重复的正相（NP）高效液相色谱（HPLC）-二极管阵列检测（DAD）方法，用于同时定性和定量测定乳基婴儿配方奶粉中的维生素A（醋酸视黄醇和棕榈酸视黄酯）和维生素E（醋酸α-生育酚、α-、γ-和δ-生育酚）。制备样品基于用乙醇进行蛋白质沉淀和维生素提取，随后用己烷再提取，而色谱方法基于使用短窄孔柱（50 mm×2.1 mm；3微米粒径），这使得溶剂消耗更少且质量灵敏度更高。该方法在精密度、回收率和灵敏度方面显示出可接受的值，并且证明对于常规分析工作非常简单。
