Taupin P, Ray J, Fischer W H, Suhr S T, Hakansson K, Grubb A, Gage F H
Laboratory of Genetics, The Salk Institute, La Jolla, California 92037, USA.
Neuron. 2000 Nov;28(2):385-97. doi: 10.1016/s0896-6273(00)00119-7.
We have purified and characterized a factor, from the conditioned medium of neural stem cell cultures, which is required for fibroblast growth factor 2's (FGF-2) mitogenic activity on neural stem cells. This autocrine/paracrine cofactor is a glycosylated form of cystatin C (CCg), whose N-glycosylation is required for its activity. We further demonstrated that, both in vitro and in vivo, neural stem cells undergoing cell division are immunopositive for cystatin C. Finally, we showed in vivo functional activity of CCg by demonstrating that the combined delivery of FGF-2 and CCg to the adult dentate gyrus stimulated neurogenesis. We propose that the process of neurogenesis is controlled by the cooperation between trophic factors and autocrine/paracrine cofactors, of which CCg is a prototype.
我们已经从神经干细胞培养的条件培养基中纯化并鉴定了一种因子，它是成纤维细胞生长因子2（FGF-2）对神经干细胞有丝分裂活性所必需的。这种自分泌/旁分泌辅因子是胱抑素C的糖基化形式（CCg），其N-糖基化对其活性是必需的。我们进一步证明，在体外和体内，正在进行细胞分裂的神经干细胞对胱抑素C呈免疫阳性。最后，我们通过证明将FGF-2和CCg联合递送至成年齿状回可刺激神经发生，展示了CCg在体内的功能活性。我们提出神经发生过程受营养因子与自分泌/旁分泌辅因子之间的协同作用控制，其中CCg是一个原型。
