Ougolkov A, Mai M, Takahashi Y, Omote K, Bilim V, Shimizu A, Minamoto T
Division of Molecular Diagnostic Pathology, Cancer Research Institute, Kanazawa University, Ishikawa, Japan.
J Exp Clin Cancer Res. 2000 Sep;19(3):349-55.
To investigate the possible relationship between altered expression (loss of membranous staining or nuclear accumulation) of beta-catenin and invasion/metastasis in early gastric cancer (EGC), beta-catenin was detected immunohistochemically in 116 cases of EGC, including 86 differentiated and 30 undifferentiated carcinomas. In parallel, immunohistochemical expression of c-erbB-2 was analyzed in all EGC cases. Regardless of histological type, altered expression of beta-catenin was found in 47% of mucosal carcinomas and 89% of carcinomas with submucosal invasion (p<0.001). Of particular interest is that beta-catenin alteration was found in almost all EGCs with lymph node metastasis, even though no significant statistical comparison could be made. These results suggest that molecular changes resulting in abnormal beta-catenin expression participate in the process of submucosal invasion and metastasis. While loss of expression was preferentially observed in undifferentiated EGCs, nuclear accumulation was found exclusively in 24% of differentiated EGCs. c-erbB-2 was overexpressed in only 16% of differentiated EGCs but there was no correlation between this overexpression and invasion or metastasis. However, it is intriguing that 12 out of 14 cases with c-erbB-2 overexpression also showed altered beta-catenin expression, suggesting that both molecules are involved in the development of a certain set of differentiated EGCs.
为了研究β-连环蛋白表达改变（膜染色缺失或核内积聚）与早期胃癌（EGC）侵袭/转移之间的可能关系，对116例EGC进行了β-连环蛋白的免疫组织化学检测，其中包括86例分化型癌和30例未分化癌。同时，对所有EGC病例分析了c-erbB-2的免疫组织化学表达。无论组织学类型如何，在47%的黏膜癌和89%的侵犯黏膜下层的癌中发现了β-连环蛋白表达改变（p<0.001）。特别值得注意的是，几乎在所有有淋巴结转移的EGC中都发现了β-连环蛋白改变，尽管无法进行显著的统计学比较。这些结果表明，导致β-连环蛋白异常表达的分子变化参与了黏膜下层侵袭和转移过程。虽然在未分化的EGC中优先观察到表达缺失，但核内积聚仅在24%的分化型EGC中发现。c-erbB-2仅在16%的分化型EGC中过表达，且这种过表达与侵袭或转移之间没有相关性。然而，有趣的是，在14例c-erbB-2过表达的病例中有12例也显示出β-连环蛋白表达改变，这表明这两种分子都参与了某些分化型EGC的发生发展。
