Socaciu C, Jessel R, Diehl H A
Department of Chemistry and Biochemistry, University of Agricultural Sciences and Vetinary Medicine, Romania.
Spectrochim Acta A Mol Biomol Spectrosc. 2000 Dec;56(14):2799-809. doi: 10.1016/s1386-1425(00)00375-9.
The carotenoids beta-carotene (BC), lycopene (LYC), lutein (LUT), zeaxanthin (ZEA), canthaxanthin (CTX) and astaxanthin (ASTA) have been incorporated into pig liver microsomes. Effective incorporation concentrations in the range of about 1-6 nmol/mg microsomal protein were obtained. A stability test at room temperature revealed that after 3 h BC and LYC had decayed totally whereas, gradually, CTX (46%), LUT (21%), ASTA (17%) and ZEA (5%) decayed. Biophysical parameters of the microsomal membrane were changed hardly by the incorporation of carotenoids. A small rigidification may occur. Membrane anisotropy seems to offer only a small tolerance for incorporation of carotenoids and seems to limit the achievable incorporation concentrations of the carotenoids into microsomes. Microsomes instead of liposomes should be preferred as a membrane model to study mutual effects of carotenoids and membrane dynamics.
类胡萝卜素β-胡萝卜素（BC）、番茄红素（LYC）、叶黄素（LUT）、玉米黄质（ZEA）、角黄素（CTX）和虾青素（ASTA）已被掺入猪肝微粒体中。获得了约1-6 nmol/mg微粒体蛋白范围内的有效掺入浓度。室温下的稳定性测试表明，3小时后BC和LYC完全降解，而CTX（46%）、LUT（21%）、ASTA（17%）和ZEA（5%）逐渐降解。类胡萝卜素的掺入对微粒体膜的生物物理参数几乎没有影响。可能会出现轻微的刚性化。膜各向异性似乎对类胡萝卜素的掺入耐受性很小，并且似乎限制了类胡萝卜素在微粒体中可达到的掺入浓度。作为研究类胡萝卜素与膜动力学相互作用的膜模型，应优先选择微粒体而非脂质体。
