Kitahara Y, Taga K, Abe H, Shimoji K
Department of Anesthesiology, Brain Research Institute, Niigata University School of Medicine, Japan.
J Neurosurg Anesthesiol. 2001 Jan;13(1):26-32. doi: 10.1097/00008506-200101000-00005.
The effects of anesthetics on the generation of cortical spreading depression (CSD) were investigated. Volatile anesthetics halothane, isoflurane, sevoflurane (0.5, 1.0, and 2.0 MAC), and the intravenous anesthetic pentobarbital were studied. Cortical spreading depression was induced by 3M-KCl applied to a surface of brain cortex for 30 minutes. Direct current (DC) potential was recorded, and the number, amplitude, and duration of CSDs were observed. With increasing concentrations of each volatile anesthetic, there was a dose-related reduction in CSD frequency but not in CSD amplitude. At 2.0 MAC of sevoflurane the suppression of CSD was less than with the other volatile anesthetics. In addition, the influence of anesthetics on expression of c-fos mRNA was investigated. Additional animals anesthetized by isoflurane or sevoflurane were studied. Five CSDs were elicited by electric stimulation (0.5 mV, 1 second) in each animal. In situ hybridization with 35S-labeled oligonucleotides was used to evaluate the level of c-fos mRNA. The expression of c-fos was observed in the hemisphere in which CSD was elicited, but there was no difference in expression of c-fos among the groups. We conclude that volatile anesthetics can induce suppression of CSD elicitation in a dose dependent manner, but that at high concentrations sevoflurane is significantly less effective than other volatile agents. Pentobarbital has the least effect on KCl-induced CSD. These data suggest that the choice of anesthetics can impact the results of studies examining membrane depolarization and the ionic changes initiated by CSD.
研究了麻醉剂对皮层扩散性抑制（CSD）产生的影响。研究了挥发性麻醉剂氟烷、异氟烷、七氟烷（0.5、1.0和2.0 MAC）以及静脉麻醉剂戊巴比妥。通过将3M - KCl应用于大脑皮层表面30分钟来诱导皮层扩散性抑制。记录直流（DC）电位，并观察CSD的数量、幅度和持续时间。随着每种挥发性麻醉剂浓度的增加，CSD频率呈剂量依赖性降低，但CSD幅度未降低。在七氟烷2.0 MAC时，对CSD的抑制作用小于其他挥发性麻醉剂。此外，研究了麻醉剂对c - fos mRNA表达的影响。研究了另外一些用异氟烷或七氟烷麻醉的动物。在每只动物中通过电刺激（0.5 mV，1秒）诱发5次CSD。使用35S标记的寡核苷酸进行原位杂交以评估c - fos mRNA的水平。在诱发CSD的半球中观察到了c - fos的表达，但各组之间c - fos的表达没有差异。我们得出结论，挥发性麻醉剂可以以剂量依赖性方式诱导对CSD诱发的抑制，但在高浓度时，七氟烷的效果明显低于其他挥发性药物。戊巴比妥对KCl诱导的CSD影响最小。这些数据表明，麻醉剂的选择可能会影响研究膜去极化和由CSD引发的离子变化的结果。
