氨基葡萄糖输注会损害大鼠运动刺激引起的葡萄糖周转。

Exercise-stimulated glucose turnover in the rat is impaired by glucosamine infusion.

作者信息

Miles P D, Higo K, Olefsky J M

机构信息

Department of Surgery, University of California-San Diego, USA.

出版信息

Diabetes. 2001 Jan;50(1):139-42. doi: 10.2337/diabetes.50.1.139.

DOI:10.2337/diabetes.50.1.139

PMID:11147779

Abstract

The infusion of glucosamine causes insulin resistance, presumably by entering the hexosamine biosynthetic pathway; it has been proposed that this pathway plays a role in hyperglycemia-induced insulin resistance. This study was undertaken to determine if glucosamine infusion could influence exercise-stimulated glucose uptake. Male SD rats were infused with glucosamine at 0.1 mg x kg(-1) x min(-1) (low-GlcN group), 6.5 mg x kg(-1) x min(-1) (high-GlcN group), or saline (control group) for 6.5 h and exercised on a treadmill for 30 min (17 m/min) at the end of the infusion period. Glucosamine infusion caused a modest increase in basal glycemia in both experimental groups, with no change in tracer-determined basal glucose turnover. During exercise, glucose turnover increased approximately 2.2-fold from 46 +/- 2 to 101 +/- 5 pmol x kg(-1) x min(-1) in the control group. Glucose turnover increased to a lesser extent in the glucosamine groups and was limited to 88% of control in the low-GlcN group (47 +/- 2 to 90 +/- 3 pmol x kg(-1) x min(-1); P < 0.01) and 72% of control in the high-GlcN group (43 +/- 1 to 73 +/- 3 pmol kg(-1) 1 min(-1); P < 0.01). Similarly, the metabolic clearance rate (MCR) in the control group increased 72% from 6.1 +/- 0.2 to 10.5 +/- 0.7 ml kg(-1) x min(-1) in response to exercise. However, the increase in MCR was only 83% of control in the low-GlcN group (5.2 +/- 0.5 to 8.7 +/- 0.5 ml x kg(-1) x min(-1); P < 0.01) and 59% of control in the high-GlcN group (4.5 +/- 0.2 to 6.2 +/- 0.3 ml x kg(-1) x min(-1); P < 0.01). Neither glucosamine infusion nor exercise significantly affected plasma insulin or free fatty acid (FFA) concentrations. In conclusion, the infusion of glucosamine, which is known to cause insulin resistance, also impaired exercise-induced glucose uptake. This inhibition was independent of hyperglycemia and FFA levels.

摘要

输注氨基葡萄糖会导致胰岛素抵抗，可能是通过进入己糖胺生物合成途径；有人提出该途径在高血糖诱导的胰岛素抵抗中起作用。本研究旨在确定输注氨基葡萄糖是否会影响运动刺激的葡萄糖摄取。雄性SD大鼠分别以0.1mg·kg⁻¹·min⁻¹（低氨基葡萄糖组）、6.5mg·kg⁻¹·min⁻¹（高氨基葡萄糖组）的剂量输注氨基葡萄糖或输注生理盐水（对照组），持续6.5小时，并在输注期结束时在跑步机上以17m/min的速度运动30分钟。输注氨基葡萄糖使两个实验组的基础血糖适度升高，示踪剂测定的基础葡萄糖周转率无变化。运动期间，对照组的葡萄糖周转率从46±2pmol·kg⁻¹·min⁻¹增加到101±5pmol·kg⁻¹·min⁻¹，约增加2.2倍。氨基葡萄糖组的葡萄糖周转率升高幅度较小，低氨基葡萄糖组限制在对照组的88%（47±2至90±3pmol·kg⁻¹·min⁻¹；P<0.01），高氨基葡萄糖组限制在对照组的72%（43±1至73±3pmol·kg⁻¹·min⁻¹；P<0.01）。同样，对照组的代谢清除率（MCR）因运动而从6.1±0.2ml·kg⁻¹·min⁻¹增加72%至10.5±0.7ml·kg⁻¹·min⁻¹。然而，低氨基葡萄糖组MCR的增加仅为对照组的83%（5.2±0.5至8.7±0.5ml·kg⁻¹·min⁻¹；P<0.01），高氨基葡萄糖组为对照组的59%（4.5±0.2至6.2±0.3ml·kg⁻¹·min⁻¹；P<0.01）。输注氨基葡萄糖和运动均未显著影响血浆胰岛素或游离脂肪酸（FFA）浓度。总之，已知会导致胰岛素抵抗的氨基葡萄糖输注也损害了运动诱导的葡萄糖摄取。这种抑制与高血糖和FFA水平无关。